Document Detail

Effects of high glucose on the hypoxic isolated guinea pig heart: interactions with ATP-dependent K+ channels?
MedLine Citation:
PMID:  10216242     Owner:  NLM     Status:  MEDLINE    
The effect of perfusion with elevated glucose concentrations on hypoxic myocardium was investigated in isolated Langendorff guinea pig hearts. For that purpose, mechanical (heart rate, systolic peak pressure and coronary flow) and electrophysiological (monophasic action potential duration=MAP, ectopic beats) data were evaluated. At the end of the experiments the hearts were examined histologically after trypan blue vital staining for quantification of irreversible myocardial damage. In the absence of insulin moderate glucose elevation (from 5 to 15 mM) exerted beneficial effects on hypoxic hearts: the depressed contraction was improved, the action potential shortening partly reversed and the percentage of irreversibly damaged myocytes diminished. Glucose did not have any effect on heart rate and arrhythmias under hypoxia or reperfusion. A contribution of cardiac ATP-dependent K+ channels to the effects of glucose could be excluded by further experiments. Thus, blocking these channels with high glibenclamide concentrations did not affect the action of glucose on MAP and contraction. To some degree the glucose effect on MAP, but not on systolic pressure, was also observable under normoxic conditions.
S Gillessen; H Kammermeier
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1427     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-06-10     Completed Date:  1999-06-10     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  256-64     Citation Subset:  IM    
Institut für Physiologie der Medizinischen Fakultät der RWTH Aachen, Universitätsklinikum, Pauwelsstr. 30, D-52057, Aachen, Germany.
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MeSH Terms
Action Potentials / drug effects
Adenosine Triphosphate / pharmacology
Cell Hypoxia
Coronary Circulation / drug effects
Dose-Response Relationship, Drug
Glucose / pharmacology*
Guinea Pigs
Heart / drug effects*,  physiology
Heart Rate / drug effects
Potassium Channels / physiology*
Trypan Blue
Reg. No./Substance:
0/Potassium Channels; 10238-21-8/Glyburide; 50-99-7/Glucose; 56-65-5/Adenosine Triphosphate; 72-57-1/Trypan Blue

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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