| Effects of a high-galactose diet on the interphotoreceptor matrix of two strains of rat. | |
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MedLine Citation:
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PMID: 8449675 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: The authors previously reported that a diet containing 30% galactose retards the development of the late-onset photoreceptor dystrophy in the spontaneously hypertensive (SHR) rat. It was suggested that the dystrophy might result from faulty galactosylation of a critical glycoprotein or glycosaminoglycan (GAG) in the interphotoreceptor matrix (IPM). In the current study, this hypothesis was tested by studying IPM protein and GAG composition in SHR and normotensive Wistar-Kyoto (WKy) control strain rats fed a standard or a high-galactose (30%) diet. METHODS: The authors performed biochemical analyses of the IPM of SHR and of WKy control rats fed either the basal diet or a 30% galactose diet for 14 mo and labeled at the termination of the experiment with 3H-glucosamine and 35S-sulfate. Analyses included high-performance liquid chromatography and two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing (IEF) of extracted proteoglycans with identification of the GAG by selective enzymatic degradation and immunoblotting of SDS-PAGE gels for interphotoreceptor retinol-binding protein (IRBP), with slot blots for additional quantitation. RESULTS: Although several differences were detected in GAG radiolabeling between the two strains, only one, a decreased overall synthesis of 3H-glucosamine-labeled GAG in the WKy rats, was altered by the high-galactose diet. There was no apparent difference in the protein patterns of the IPM as evaluated by two-dimensional SDS-PAGE and IEF. However, slot blots of that portion of the IPM extracted from the neural retinas showed less reactivity per microgram of protein for IRBP in the galactose-fed animals of both strains than for the rats fed a basal diet. This was statistically significant, however, only in the WKy rats. CONCLUSIONS: The composition of the IPM of these two strains of rat appears similar to the composition of the IPM of other species that have been studied. Whether the quantitative alteration in IRBP that appears to be produced by galactose feeding has functional significance, with particular relevance to retarding the development of the photoreceptor cell dystrophy of the SHR rat, is unclear at this time. |
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Authors:
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A Kennedy; R N Frank |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: 34 ISSN: 0146-0404 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 1993 Mar |
Date Detail:
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Created Date: 1993-04-12 Completed Date: 1993-04-12 Revised Date: 2010-03-24 |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 547-58 Citation Subset: IM |
Affiliation:
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Kresge Eye Institute, Wayne State University, School of Medicine, Detroit, Michigan 48201. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Choroid / metabolism Chromatography, High Pressure Liquid Diet Electrophoresis, Gel, Two-Dimensional Extracellular Matrix / metabolism* Eye Proteins* Galactose / administration & dosage* Glycosaminoglycans / metabolism* Hypertension / metabolism Isoelectric Point Male Photoreceptor Cells / metabolism* Rats Rats, Inbred SHR Rats, Inbred WKY Retinal Degeneration / metabolism Retinol-Binding Proteins / metabolism Sclera / metabolism Species Specificity |
| Grant Support | |
ID/Acronym/Agency:
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R01-01857//PHS HHS; R01-02566//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Eye Proteins; 0/Glycosaminoglycans; 0/Retinol-Binding Proteins; 0/interstitial retinol-binding protein; 26566-61-0/Galactose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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