Document Detail


Effects of high fat provision on muscle PDH activation and malonyl-CoA content in moderate exercise.
MedLine Citation:
PMID:  11090589     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study examined the effects of elevated free fatty acid (FFA) provision on the regulation of pyruvate dehydrogenase (PDH) activity and malonyl-CoA (M-CoA) content in human skeletal muscle during moderate-intensity exercise. Seven men rested for 30 min and cycled for 10 min at 40% and 10 min at 65% of maximal O(2) uptake while being infused with either Intralipid and heparin (Int) or saline (control). Muscle biopsies were taken at 0, 1 (rest-to-exercise transition), 10, and 20 min. Exercise plasma FFA were elevated (0.99 +/- 0.11 vs. 0.33 +/- 0.03 mM), and the respiratory exchange ratio was reduced during Int (0.87 +/- 0.02) vs. control (0.91 +/- 0.01). PDH activation was lower during Int at 1 min (1.33 +/- 0.19 vs. 2.07 +/- 0.14 mmol. min(-1). kg(-1) wet muscle) and throughout exercise. Muscle pyruvate was reduced during Int at rest [0.17 +/- 0.03 vs. 0.25 +/- 0.03 mmol/kg dry muscle (dm)] but increased above control during exercise. NADH was higher during Int vs. control at rest and 1 min of exercise (0.122 +/- 0.016 vs. 0.102 +/- 0.005 and 0.182 +/- 0.016 vs. 0.150 +/- 0.016 mmol/kg dm), but not at 10 and 20 min. M-CoA was lower during Int vs. control at rest and 20 min of exercise (1.12 +/- 0.22 vs. 1.43 +/- 0.17 and 1.33 +/- 0.16 vs. 1.84 +/- 0.17 micromol/kg dm). The reduced PDH activation with elevated FFA during the rest-to-exercise transition was related to higher mitochondrial NADH at rest and 1 min of exercise and lower muscle pyruvate at rest. The decreased M-CoA may have increased fat oxidation during exercise with elevated FFA by reducing carnitine palmitoyltransferase I inhibition and increasing mitochondrial FFA transport.
Authors:
L M Odland; G J Heigenhauser; L L Spriet
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  89     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2000-12-26     Completed Date:  2001-01-11     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2352-8     Citation Subset:  IM    
Affiliation:
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
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MeSH Terms
Descriptor/Qualifier:
Adult
Dietary Fats / administration & dosage*,  pharmacology
Enzyme Activation / drug effects
Exercise / physiology*
Fat Emulsions, Intravenous / administration & dosage,  pharmacology
Heparin / administration & dosage,  pharmacology
Humans
Infusions, Intravenous
Male
Malonyl Coenzyme A / metabolism*
Muscle, Skeletal / enzymology*,  metabolism
Pulmonary Gas Exchange
Pyruvate Dehydrogenase Complex / metabolism*
Pyruvic Acid / metabolism
Sodium Chloride / administration & dosage,  pharmacology
Time Factors
Chemical
Reg. No./Substance:
0/Dietary Fats; 0/Fat Emulsions, Intravenous; 0/Pyruvate Dehydrogenase Complex; 127-17-3/Pyruvic Acid; 524-14-1/Malonyl Coenzyme A; 7647-14-5/Sodium Chloride; 9005-49-6/Heparin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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