Document Detail


Effects of herbal medicinal products and food supplements on induction of CYP1A2, CYP3A4 and MDR1 in the human colon carcinoma cell line LS180.
MedLine Citation:
PMID:  17163579     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A selection of popular herbal medicinal products and food supplements were analysed for their potential to modulate the expression of the cytochrome P450 enzymes CYP1A2 and CYP3A4 and the transporter protein MDR1. A total of 31 products were analysed. Nine of the products have been approved by the Medical Products Agency (MPA) in Sweden and are marketed as herbal medicinal products. Twenty-two of the products have not been assessed by the MPA and are marketed as food supplements. LS180 cells were exposed to extracts from the different herbal products and real-time quantitative polymerase chain reaction, RT-QPCR, was subsequently used to analyse the relative mRNA levels of CYP1A2, CYP3A4 or MDR1 in treated and non-treated cells. Our results show that 17 of 31 products tested induced a two-fold expression or more for at least one of the genes analysed. Four products, of which a ginger-supplement was the most potent, induced all three genes.
Authors:
Helena Brandin; Eila Viitanen; Olle Myrberg; Ann-Kristin Arvidsson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Phytotherapy research : PTR     Volume:  21     ISSN:  0951-418X     ISO Abbreviation:  Phytother Res     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-08     Completed Date:  2007-05-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8904486     Medline TA:  Phytother Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  239-44     Citation Subset:  IM    
Affiliation:
Medical Products Agency, Uppsala, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor / enzymology
Colonic Neoplasms / enzymology
Cytochrome P-450 CYP1A2 / biosynthesis,  genetics
Cytochrome P-450 CYP3A
Cytochrome P-450 Enzyme System / biosynthesis*,  genetics
DNA Primers
Dietary Supplements*
Humans
P-Glycoprotein / biosynthesis*,  genetics
Phytotherapy*
Plants, Medicinal*
RNA / analysis
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/DNA Primers; 0/P-Glycoprotein; 63231-63-0/RNA; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.14.13.67/CYP3A4 protein, human; EC 1.14.14.1/Cytochrome P-450 CYP1A2; EC 1.14.14.1/Cytochrome P-450 CYP3A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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