Document Detail


Effects of hemoglobin concentration on deformability of individual sickle cells after deoxygenation.
MedLine Citation:
PMID:  7718897     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To assess the role of intracellular hemoglobin concentration in the deformability of sickle (HbSS) cells after deoxygenation, rheologic coefficients (static rigidity E and dynamic rigidity eta) of density-fractionated individual sickle erythrocytes (SS cells) were determined as a function of oxygen tension (pO2) using the micropipette technique in a newly developed experimental chamber. With stepwise deoxygenation, E and eta values showed no significant increase before morphologic sickling but rose sharply after sickling. In denser cells, continued deoxygenation led to steep rises of E and eta toward infinity, as the cell behaved as a solid. The pO2 levels at which rheologic and morphologic changes occurred for individual SS cells during deoxygenation varied directly with the cell density. The extent of recovery in E and eta during reoxygenation varied inversely with the cell density. These results provide direct evidence that the intracellular sickle hemoglobin (HbS) concentration of SS cells plays an important role in their rheologic heterogeneity in deoxygenation and reoxygenation. The elevations of eta during pO2 alteration were greater than those of E, especially for the denser cells, suggesting the importance of the elevated dynamic rigidity in initiating microcirculatory disturbances in sickle cell disease.
Authors:
T Itoh; S Chien; S Usami
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Blood     Volume:  85     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1995-05-25     Completed Date:  1995-05-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2245-53     Citation Subset:  AIM; IM    
Affiliation:
Department of Physiology and Cellular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY.
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MeSH Terms
Descriptor/Qualifier:
Anemia, Sickle Cell / blood*,  pathology
Erythrocyte Deformability*
Hemoglobin, Sickle / analysis*
Hemorheology
Humans
Oxygen / blood*
Partial Pressure
Grant Support
ID/Acronym/Agency:
HL28381/HL/NHLBI NIH HHS; HL43026/HL/NHLBI NIH HHS; HL44147/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Hemoglobin, Sickle; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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