| Effects of hemagglutinin-neuraminidase protein mutations on cell-cell fusion mediated by human parainfluenza type 2 virus. | |
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MedLine Citation:
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PMID: 18562539 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The monoclonal antibody M1-1A, specific for the hemagglutinin-neuraminidase (HN) protein of human parainfluenza type 2 virus (HPIV2), blocks virus-induced cell-cell fusion without affecting the hemagglutinating and neuraminidase activities. F13 is a neutralization escape variant selected with M1-1A and contains amino acid mutations N83Y and M186I in the HN protein, with no mutation in the fusion protein. Intriguingly, F13 exhibits reduced ability to induce cell-cell fusion despite its multistep replication. To investigate the potential role of HPIV2 HN protein in the regulation of cell-cell fusion, we introduced these mutations individually or in combination to the HN protein in the context of recombinant HPIV2. Following infection at a low multiplicity, Vero cells infected with the mutant virus H-83/186, which carried both the N83Y and M186I mutations, remained as nonfused single cells at least for 24 h, whereas most of the cells infected with wild-type virus mediated prominent cell-cell fusion within 24 h. On the other hand, the cells infected with the mutant virus, carrying either the H-83 or H-186 mutation, mediated cell-cell fusion but less efficiently than those infected with wild-type virus. Irrespective of the ability to cause cell-cell fusion, however, every virus could infect all the cells in the culture within 48 h after the initial infection. These results indicated that both the N83Y and M186I mutations in the HN protein are involved in the regulation of cell-cell fusion. Notably, the limited cell-cell fusion by H-83/186 virus was greatly promoted by lysophosphatidic acid, a stimulator of the Ras and Rho family GTPases. |
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Authors:
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Masato Tsurudome; Machiko Nishio; Morihiro Ito; Shunsuke Tanahashi; Mitsuo Kawano; Hiroshi Komada; Yasuhiko Ito |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-06-18 |
Journal Detail:
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Title: Journal of virology Volume: 82 ISSN: 1098-5514 ISO Abbreviation: J. Virol. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-08-15 Completed Date: 2008-09-29 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: United States |
Other Details:
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Languages: eng Pagination: 8283-95 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507 Japan. turudome@doc.medic.mie-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Fusion* Cercopithecus aethiops HN Protein / genetics*, metabolism, physiology Humans Mutagenesis, Site-Directed Mutation* Parainfluenza Virus 2, Human / physiology* Recombinant Proteins / metabolism Vero Cells |
| Chemical | |
Reg. No./Substance:
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0/HN Protein; 0/Recombinant Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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