Document Detail


Effects of heat inactivation on HIV antibody screening and confirmatory test systems.
MedLine Citation:
PMID:  3066568     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to evaluate the effect of heat inactivation on serum undergoing testing for HIV antibody, 100 heat-inactivated and nonheat-inactivated serum samples were tested by two modifications of Abbott's screening assays for human T-cell lymphotropic virus type-III (lot numbers 1037 and 3036) and by two confirmatory assays (Cambridge BioScience CBre3-EIA; Damon Corporation Western blot). The samples consisted of 75 HIV antibody-negative and 25 HIV antibody-positive sera. The specimens were divided into two equal aliquots. One set was not subjected to heat inactivation, while the others were subjected to heat inactivation at 56 degrees C for 30 min. Heat inactivation had no significant effect on the HIV-position sera; however, heat-inactivated, negative sera evaluated by Abbott lot numbers 1037 and 3036 resulted in false-positive rates of 8% and 7%, respectively. No false positives were generated by the two confirmatory assays; however, the CBre3-EIA recombinant envelope protein assay had a significantly increased optical density reading following heat inactivation of the negative sera. The Western blot procedure used in the study was not affected by heat inactivation.
Authors:
D R Fipps; J J Damato; D S Burke
Related Documents :
3897258 - Evaluation of commercial methods of enzyme immunoassay (eia) for the measurement of rub...
15135738 - Virological exploration of individuals with discordant hiv screening tests.
18814068 - Twenty-two years of failure to set up undisputed assays to detect patients with the ant...
16249658 - Evaluation of rapid tests for anti-hiv detection in brazil.
8790158 - Development of anti-interferon antibodies and breakthrough hepatitis during treatment f...
2070558 - Quantification of cross-reactive idiotype-positive rheumatoid factor produced in autoim...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Diagnostic microbiology and infectious disease     Volume:  10     ISSN:  0732-8893     ISO Abbreviation:  Diagn. Microbiol. Infect. Dis.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1989-04-06     Completed Date:  1989-04-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8305899     Medline TA:  Diagn Microbiol Infect Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  103-7     Citation Subset:  IM; X    
Affiliation:
Department of Virus Disease, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, DC 20307-5100.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
AIDS Serodiagnosis / standards
Blotting, Western
Drug Stability
False Positive Reactions
HIV Antibodies / analysis*
Hot Temperature
Humans
Immunoenzyme Techniques
Chemical
Reg. No./Substance:
0/HIV Antibodies

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Random blood glucose sampling as an early antenatal screening test for diabetes mellitus.
Next Document:  Primary lymphocutaneous Nocardia brasiliensis infection: three case reports and a review of the lite...