Document Detail

Effects of heat inactivation on HIV antibody screening and confirmatory test systems.
MedLine Citation:
PMID:  3066568     Owner:  NLM     Status:  MEDLINE    
In order to evaluate the effect of heat inactivation on serum undergoing testing for HIV antibody, 100 heat-inactivated and nonheat-inactivated serum samples were tested by two modifications of Abbott's screening assays for human T-cell lymphotropic virus type-III (lot numbers 1037 and 3036) and by two confirmatory assays (Cambridge BioScience CBre3-EIA; Damon Corporation Western blot). The samples consisted of 75 HIV antibody-negative and 25 HIV antibody-positive sera. The specimens were divided into two equal aliquots. One set was not subjected to heat inactivation, while the others were subjected to heat inactivation at 56 degrees C for 30 min. Heat inactivation had no significant effect on the HIV-position sera; however, heat-inactivated, negative sera evaluated by Abbott lot numbers 1037 and 3036 resulted in false-positive rates of 8% and 7%, respectively. No false positives were generated by the two confirmatory assays; however, the CBre3-EIA recombinant envelope protein assay had a significantly increased optical density reading following heat inactivation of the negative sera. The Western blot procedure used in the study was not affected by heat inactivation.
D R Fipps; J J Damato; D S Burke
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Diagnostic microbiology and infectious disease     Volume:  10     ISSN:  0732-8893     ISO Abbreviation:  Diagn. Microbiol. Infect. Dis.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1989-04-06     Completed Date:  1989-04-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8305899     Medline TA:  Diagn Microbiol Infect Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  103-7     Citation Subset:  IM; X    
Department of Virus Disease, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, DC 20307-5100.
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MeSH Terms
AIDS Serodiagnosis / standards
Blotting, Western
Drug Stability
False Positive Reactions
HIV Antibodies / analysis*
Hot Temperature
Immunoenzyme Techniques
Reg. No./Substance:
0/HIV Antibodies

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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