Document Detail


Effects of growth temperature on the fatty acid composition of the free-living nematode Caenorhabditis elegans.
MedLine Citation:
PMID:  8934450     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of growth temperature on the fatty acid compositions of the phosphatidylcholine (PC), phosphatidylethanolamine (PE), and total lipid (TL) fractions of the free-living nematode Caenorhabditis elegans were investigated. A reduction in growth temperature from 25 to 15 degrees C caused the proportions of eicosapentaenoic acid (20:5n-3) to increase from 23.6 to 32.5% in the PC, from 7.4 to 10.8% in the PE, and from 12.9 to 19.9% in the TL fractions. Conversely, the levels of dihomo-gamma-linolenic acid (20:3n-6) and arachidonic acid (20:4n-6) in these phospholipid fractions and the TL fraction both decreased with decreasing growth temperature. Analysis of the positional distribution of fatty acids in the PC fraction revealed that the change in the composition of C20 polyunsaturated fatty acid was obvious in position sn-2. Lowering the growth temperature induced an increase in the level of the diacyl subclass of PE from 58% at 25 degrees C to 71% at 15 degrees C, with a concomitant decrease in the levels of the alkylacyl and alkenylacyl subclass of PE of C. elegans. These changes observed in the phospholipids of C. elegans might be one mechanism for adaptation to low temperature.
Authors:
T Tanaka; K Ikita; T Ashida; Y Motoyama; Y Yamaguchi; K Satouchi
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Lipids     Volume:  31     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1997-03-28     Completed Date:  1997-03-28     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1173-8     Citation Subset:  IM    
Affiliation:
Department of Food Science and Technology, Fukuyama University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological
Animals
Caenorhabditis elegans / growth & development,  metabolism*
Diet
Eicosapentaenoic Acid / metabolism
Escherichia coli
Fatty Acids / chemistry,  metabolism*
Phosphatidylcholines / chemistry,  metabolism
Phosphatidylethanolamines / chemistry,  metabolism
Phospholipids / chemistry,  classification,  metabolism
Temperature
Triglycerides / chemistry,  metabolism
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Phospholipids; 0/Triglycerides; 1553-41-9/Eicosapentaenoic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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