Document Detail


Effects of growth temperature on fatty acid and alk-1-enyl group compositions of Veillonella parvula and Megasphaera elsdenii phospholipids.
MedLine Citation:
PMID:  7056696     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Veillonella parvula ATCC 10790, an anaerobic gram-negative coccus, contains diacyl and alk-1-enyl acyl (plasmalogen) forms of phosphatidylethanolamine and phosphatidylserine. We studied the effect of growth temperature on the lipid composition of this strain. There was a small increase in the phosphatidylethanolamine content but no change in the content of plasmalogens at the lower growth temperatures tested. The total acyl chains and the plasmalogen acyl chains contained between 73 and 80% mono-unsaturated fatty acids at all growth temperatures. The plasmalogen alk-1-enyl chains were significantly more unsaturated in cells grown at 30 and 25 degrees C than in cells grown at 37 degrees C. Differential scanning calorimetry of the hydrated phospholipids showed lower phase transition temperatures for the lipids from the cells grown at the lower temperatures. In Megasphaera elsdenii lipids, which are similar in composition to the lipids of V. parvula, the proportion of phosphatidylethanolamine also increased slightly at lower growth temperatures, with no significant change in the content of plasmalogens. M. elsdenii contained cyclopropane fatty acyl and alk-1-enyl chains in addition to the mono-unsaturated and saturated chains previously reported. As cells entered the stationary phase of growth at 30 and 42.5 degrees C, there was a reciprocal increase in the proportion of cyclopropane acyl chains and decrease in the unsaturated moieties. The total proportion of cyclopropane and unsaturated acyl and alk-1-enyl chains was more than 65% at all growth temperatures studied, and there was no discernible increase in the sum of these moieties at the lower growth temperatures.
Authors:
N C Johnston; H Goldfine
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  149     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1982 Feb 
Date Detail:
Created Date:  1982-04-20     Completed Date:  1982-04-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  567-75     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acidaminococcaceae / analysis*,  growth & development
Fatty Acids / analysis
Membrane Lipids / analysis
Phosphatidylethanolamines / analysis
Phosphatidylserines / analysis
Phospholipids / analysis*
Plasmalogens / analysis*
Temperature
Veillonella / analysis*,  growth & development
Grant Support
ID/Acronym/Agency:
AI08903/AI/NIAID NIH HHS; RR542/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Membrane Lipids; 0/Phosphatidylethanolamines; 0/Phosphatidylserines; 0/Phospholipids; 0/Plasmalogens
Comments/Corrections

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