Document Detail

Effects of growth factors on cell migration and alkaline phosphatase release.
MedLine Citation:
PMID:  16817586     Owner:  NLM     Status:  MEDLINE    
Segmental defects in long bones pose a clinical challenge in orthopaedic surgery. These defects often require multiple surgeries to achieve the desired bridging and union. Bone morphogenetic protein 2 (BMP-2) has been used extensively to facilitate regeneration in these large defects. The two important roles that BMP-2 has to play in large defect regeneration are chemotaxis to bone cells to migrate and induction of bone matrix production. Experiments were designed to study the effect of BMP-2 on alkaline phosphatase (ALP) production in cells that have migrated through a porous membrane in a cell migration chamber under the influence of culture media supplemented with BMP-2. Two cell lines, UMR cells and MC3T3-E1 cells, were used. A modified Boyden chamber with 8 microm membrane filter was used. Cell migration under the influence of BMP-2 concentration of 1, 10, 100, and 1000 ng/ml was studied. In both cells, the highest chemotactic effect was observed at 10 ng/ml. The ALP activity in the migrated cell was then characterized. A pattern of increase in ALP activity with increased BMP-2 dose was observed in the migrated cells.
Krista N Gonnerman; Lauren S Brown; Tien-Min G Chu
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomedical sciences instrumentation     Volume:  42     ISSN:  0067-8856     ISO Abbreviation:  Biomed Sci Instrum     Publication Date:  2006  
Date Detail:
Created Date:  2006-07-04     Completed Date:  2006-07-27     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0140524     Medline TA:  Biomed Sci Instrum     Country:  United States    
Other Details:
Languages:  eng     Pagination:  60-5     Citation Subset:  IM    
Department of Biomedical Engineering, Indiana University-Purdue University Indianapolis, 1120 South Drive, Rm. 146, Indianapolis, IN 46202, USA.
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MeSH Terms
Alkaline Phosphatase / metabolism*
Bone Matrix / drug effects,  physiology*
Bone Morphogenetic Protein 2
Bone Morphogenetic Proteins / administration & dosage*
Cell Differentiation / physiology
Cell Line
Cell Movement / drug effects*
Cell Survival / drug effects
Dose-Response Relationship, Drug
Enzyme Activation / drug effects
Insulin-Like Growth Factor II
Osteoblasts / cytology*,  drug effects,  physiology*
Proteins / administration & dosage
Transforming Growth Factor beta / administration & dosage*
Reg. No./Substance:
0/Bmp2 protein, mouse; 0/Bone Morphogenetic Protein 2; 0/Bone Morphogenetic Proteins; 0/IGF2 protein, human; 0/Proteins; 0/Transforming Growth Factor beta; 67763-97-7/Insulin-Like Growth Factor II; EC Phosphatase

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