Document Detail


Effects of growth conditions on the lipid composition of Bifidobacterium bifidum subsp. pennsylvanicum.
MedLine Citation:
PMID:  596859     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipid-phosphorus and lipid-galactose content and phospholipid and fatty acid composition of Bifidobacterium bifidum subsp. pennsylvanicum were examined under a wide variety of growth conditions. Cells from 29-C cultures contained less lipid-phosphorus than did cells from 37-C cultures, but their lipid-galactose content and phospholipid composition did not differ. At both temperatures, the growth phase influenced the lipid composition similarly. Phosphate, Mg2+ and K+ concentrations in the medium did neither significantly change the cellular lipid-phosphorus content nor the phospholipid composition. Only Mg2+-deficiency markedly reduced growth and lowered the content of cellular lipid-galactose. Omission of Tween 80 from the medium did not affect growth, but lowered the content of lipid-galactose and augmented those of lipid-phosphorus and diphosphatidylglycerol in the cell. Increased osmolarity and substitution of other Tween for Tween 80 caused the same changes in lipid composition, and besides inhibited growth. Omitting Tween 80 and replacing it by other Tweens dramatically reduced the percentage of unsaturated fatty acids. C12- and C14-fatty acids made up about 50% of total fatty acids in cells from Tween 20 cultures and 12-14% in cells from Tween 40 and Tween 60 cultures. The differences in the decline of unsaturated fatty acids and in the degree of replacement of these acids by C12- and C14-fatty acids may be related to the variations in growth in cultures with various Tweens by way of changes in the physical state of the membrane lipids.
Authors:
J H Veerkamp
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Antonie van Leeuwenhoek     Volume:  43     ISSN:  0003-6072     ISO Abbreviation:  Antonie Van Leeuwenhoek     Publication Date:  1977  
Date Detail:
Created Date:  1978-02-18     Completed Date:  1978-02-18     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0372625     Medline TA:  Antonie Van Leeuwenhoek     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  101-10     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Actinomycetaceae / analysis*,  drug effects,  growth & development
Culture Media
Fatty Acids, Nonesterified / pharmacology
Galactose / analysis*
Lipids / analysis*
Phospholipids / analysis*
Polysorbates / pharmacology
Temperature
Chemical
Reg. No./Substance:
0/Culture Media; 0/Fatty Acids, Nonesterified; 0/Lipids; 0/Phospholipids; 0/Polysorbates; 26566-61-0/Galactose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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