Document Detail

Effects of gonadal hormones on the peripheral cannabinoid receptor 1 (CB1R) system under a myositis condition in rats.
MedLine Citation:
PMID:  22940464     Owner:  NLM     Status:  MEDLINE    
In this study, we assessed the effects of peripherally administered cannabinoids in an orofacial myositis model, and the role of sex hormones in cannabinoid receptor (CBR) expression in trigeminal ganglia (TG). Peripherally administered arachidonylcyclopropylamide (ACPA), a specific CB1R agonist, significantly attenuated complete Freund's adjuvant (CFA)-induced mechanical hypersensitivity in the masseter muscle in male rats. The ACPA effect was blocked by a local administration of AM251, a specific CB1R antagonist, but not by AM630, a specific CB2R antagonist. In female rats, a 30-fold higher dose of ACPA was required to produce a moderate reduction in mechanical hypersensitivity. CFA injected in masseter muscle significantly upregulated CB1R mRNA expression in TG in male, but not in female, rats. There was a close correlation between the CB1R mRNA levels in TG and the antihyperalgesic effect of ACPA. Interleukin (IL)-1β and IL-6, which are elevated in the muscle tissue following CFA treatment, induced a significant upregulation of CB1R mRNA expression in TG from male rats. The upregulation of CB1R was prevented in TG cultures from orchidectomized male rats, which was restored by the application of testosterone. The cytokines did not alter the CB1R mRNA level in TG from intact as well as ovariectomized female rats. Neither estradiol supplement nor estrogen receptor blockade had any effects on CB1R expression. These data indicate that testosterone, but not estradiol, is required for the regulation of CB1Rs in TG under inflammatory conditions, which provide explanations for the sex differences in the antihyperalgesic effects of peripherally administered cannabinoids.
Katelyn Y Niu; Youping Zhang; Jin Y Ro
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural     Date:  2012-08-31
Journal Detail:
Title:  Pain     Volume:  153     ISSN:  1872-6623     ISO Abbreviation:  Pain     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-15     Completed Date:  2013-07-15     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  7508686     Medline TA:  Pain     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  2283-91     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.
Program in Neuroscience, Department of Neural and Pain Sciences, University of Maryland School of Dentistry, Baltimore, MD 21201, USA.
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MeSH Terms
Disease Models, Animal
Estrogens / physiology*
Facial Pain / drug therapy,  physiopathology*
Myositis / drug therapy,  physiopathology*
Primary Cell Culture
Rats, Sprague-Dawley
Receptor, Cannabinoid, CB1 / agonists,  antagonists & inhibitors,  genetics*
Receptor, Cannabinoid, CB2 / agonists,  antagonists & inhibitors,  physiology
Testosterone / physiology*
Grant Support
Reg. No./Substance:
0/Cnr2 protein, rat; 0/Estrogens; 0/Receptor, Cannabinoid, CB1; 0/Receptor, Cannabinoid, CB2; 58-22-0/Testosterone

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