| Effects of glycogen stores and non-esterified fatty acid availability on insulin-stimulated glucose metabolism and tissue pyruvate dehydrogenase activity in the rat. | |
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MedLine Citation:
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PMID: 2065855 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of increased tissue glycogen stores on insulin sensitivity, and on the response of insulin-stimulated glucose utilisation to an acute elevation in plasma fatty acid levels (approximately 1.5 mmol/l), were investigated in conscious rats using the hyperinsulinaemic euglycaemic clamp. Studies were performed in two groups of rats; (a) fasted 24 h; (b) fasted 4.5 h, but infused with glucose for 4 h (0.5 g/h) of this period before the clamp (fed, glucose infused rats). Clamp glucose requirement and 3-3H-glucose turnover were 20-25% lower in the fed, glucose-infused rats. In these rats, elevation of plasma fatty acid levels resulted in impaired suppression of hepatic glucose output (residual hepatic glucose output: 41 +/- 4 vs 8 +/- 6 mumol.min-1.kg-1, p less than 0.001) but did not further decrease 3-3H-glucose turnover. Elevated non-esterified fatty acid levels had no significant effect on glucose kinetics in 24 h fasted rats. In the fed glucose-infused rats, at low plasma fatty acid levels, there was no deposition of glycogen in muscle during the clamp and liver glycogen levels fell. With elevation of non-esterified fatty acid levels muscle glycogen deposition was stimulated in both groups, and there was no fall in liver glycogen during the clamps in the fed glucose-infused rats. Increased non-esterified fatty acid availability during the clamps decreased pyruvate dehydrogenase activity in liver, heart, adipose tissue and quadriceps muscle, in both groups of rats. The findings are consistent with an inhibition of glycolysis in liver, skeletal muscle and heart by increased fatty acid availability.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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Y T Kruszynska; J G McCormack; N McIntyre |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Diabetologia Volume: 34 ISSN: 0012-186X ISO Abbreviation: Diabetologia Publication Date: 1991 Apr |
Date Detail:
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Created Date: 1991-08-13 Completed Date: 1991-08-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0006777 Medline TA: Diabetologia Country: GERMANY |
Other Details:
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Languages: eng Pagination: 205-11 Citation Subset: IM |
Affiliation:
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Department of Medicine, Royal Free Hospital School of Medicine, London, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Glucose / metabolism Fat Emulsions, Intravenous / pharmacology Fatty Acids, Nonesterified / metabolism* Glucose / metabolism* Glucose Clamp Technique Glycogen / metabolism* Insulin / blood, pharmacology* Kinetics Liver Glycogen / metabolism Male Muscles / drug effects, metabolism Myocardium / metabolism Pyruvate Dehydrogenase Complex / metabolism* Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 0/Fat Emulsions, Intravenous; 0/Fatty Acids, Nonesterified; 0/Liver Glycogen; 0/Pyruvate Dehydrogenase Complex; 11061-68-0/Insulin; 50-99-7/Glucose; 9005-79-2/Glycogen |
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