Document Detail

Effects of glycerol on turkey sperm cell viability and fertilizing capacity.
MedLine Citation:
PMID:  605051     Owner:  NLM     Status:  MEDLINE    
To test the cryoprotective action of glycerol, intravaginal (I.V.A.I.) and intramagnal (I.M.A.I.) artificial inseminations were performed on 100 turkey hens using semen treated with glycerol; treated with glycerol then diluted and centrifuged; treated with glycerol and frozen-thawed; treated with glycerol, frozen-thawed then diluted and centrifuged; and untreated control. With I.V.A.I. no progeny were obtained from any turkeys within the treated group. I.V.A.I. subsequent to semen dilution and centrifugation to lower the glycerol level yielded only two poults from one of the 36 hens inseminated. When I.M.A.I. was used, many poults were hatched from eggs produced by hens in the four treated groups. To study the cause of glycerol's antifertility action, histological sections of uterovaginal junction (UVJ) and infundibular (INF) tissues were observed. Following I.V.A.I., no sperm cells were found in either the UVJ or INF regions except in the controls where they were readily seen in the UVJ region. Conversely with I.M.A.I., sperm cells were seen in the INF region in all treatment groups while in the controls, sperm cells were also found in the UVJ region.
B J Marquez; F X Ogasawara
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Poultry science     Volume:  56     ISSN:  0032-5791     ISO Abbreviation:  Poult. Sci.     Publication Date:  1977 May 
Date Detail:
Created Date:  1978-04-17     Completed Date:  1978-04-17     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0401150     Medline TA:  Poult Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  725-31     Citation Subset:  IM    
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MeSH Terms
Cell Survival
Fertilization / drug effects*
Glycerol / pharmacology*
Insemination, Artificial / veterinary
Semen Preservation / veterinary
Spermatozoa / drug effects*
Turkeys / physiology*
Reg. No./Substance:

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