| Effects of glucosinolate-rich broccoli sprouts on urinary levels of aflatoxin-DNA adducts and phenanthrene tetraols in a randomized clinical trial in He Zuo township, Qidong, People's Republic of China. | |
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MedLine Citation:
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PMID: 16284385 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Residents of Qidong, People's Republic of China, are at high risk for development of hepatocellular carcinoma, in part due to consumption of aflatoxin-contaminated foods, and are exposed to high levels of phenanthrene, a sentinel of hydrocarbon air toxics. Cruciferous vegetables, such as broccoli, contain anticarcinogens. Glucoraphanin, the principal glucosinolate in broccoli sprouts, can be hydrolyzed by gut microflora to sulforaphane, a potent inducer of carcinogen detoxication enzymes. In a randomized, placebo-controlled chemoprevention trial, we tested whether drinking hot water infusions of 3-day-old broccoli sprouts, containing defined concentrations of glucosinolates, could alter the disposition of aflatoxin and phenanthrene. Two hundred healthy adults drank infusions containing either 400 or < 3 micromol glucoraphanin nightly for 2 weeks. Adherence to the study protocol was outstanding; no problems with safety or tolerance were noted. Urinary levels of aflatoxin-N(7)-guanine were not different between the two intervention arms (P = 0.68). However, measurement of urinary levels of dithiocarbamates (sulforaphane metabolites) indicated striking interindividual differences in bioavailability. An inverse association was observed for excretion of dithiocarbamates and aflatoxin-DNA adducts (P = 0.002; R = 0.31) in individuals receiving broccoli sprout glucosinolates. Moreover, trans, anti-phenanthrene tetraol, a metabolite of the combustion product phenanthrene, was detected in urine of all participants and showed a robust inverse association with dithiocarbamate levels (P = 0.0001; R = 0.39), although again no overall difference between intervention arms was observed (P = 0.29). Understanding factors influencing glucosinolate hydrolysis and bioavailability will be required for optimal use of broccoli sprouts in human interventions. |
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Authors:
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Thomas W Kensler; Jian-Guo Chen; Patricia A Egner; Jed W Fahey; Lisa P Jacobson; Katherine K Stephenson; Lingxiang Ye; Jamie L Coady; Jin-Bing Wang; Yan Wu; Yan Sun; Qi-Nan Zhang; Bao-Chu Zhang; Yuan-Rong Zhu; Geng-Sun Qian; Stephen G Carmella; Stephen S Hecht; Lorie Benning; Stephen J Gange; John D Groopman; Paul Talalay |
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Publication Detail:
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Type: Journal Article; Randomized Controlled Trial; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology Volume: 14 ISSN: 1055-9965 ISO Abbreviation: Cancer Epidemiol. Biomarkers Prev. Publication Date: 2005 Nov |
Date Detail:
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Created Date: 2005-11-14 Completed Date: 2006-01-10 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9200608 Medline TA: Cancer Epidemiol Biomarkers Prev Country: United States |
Other Details:
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Languages: eng Pagination: 2605-13 Citation Subset: IM |
Affiliation:
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Department of Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, Room E7541, 615 North Wolfe Street, Baltimore, MD 21205, USA. tkensler@jhsph.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aflatoxins / metabolism, urine* Aged Anticarcinogenic Agents / pharmacology* Beverages Biological Availability Brassica / chemistry* Carcinoma, Hepatocellular / etiology, prevention & control DNA Adducts / urine* Female Glucosinolates / pharmacology* Humans Hydrolysis Liver Neoplasms / etiology, prevention & control Male Middle Aged Phenanthrenes / urine* Placebos |
| Grant Support | |
ID/Acronym/Agency:
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ES 03819/ES/NIEHS NIH HHS; P01 ES 06052/ES/NIEHS NIH HHS; R01 CA 92025/CA/NCI NIH HHS; R01 CA 93780/CA/NCI NIH HHS; R21 CA 098485/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Aflatoxins; 0/Anticarcinogenic Agents; 0/DNA Adducts; 0/Glucosinolates; 0/Phenanthrenes; 0/Placebos |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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