Document Detail


Effects of ginseng saponins isolated from red ginseng on ultraviolet B-induced skin aging in hairless mice.
MedLine Citation:
PMID:  19041641     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It is well-known that chronic ultraviolet B (UVB) exposure at low-dose causes skin photoaging including increases in skin thickness and wrinkle formation and reduction in skin elasticity. This study examined the effects of total saponins and ginsenoside Rb(1) isolated from Red Ginseng roots on skin thickness, elasticity, and wrinkle formation caused by long-term, low-dose UVB irradiation in hairless mice. The topical application of total ginseng saponins (10 pg or 100 ng/mouse) and ginsenoside Rb(1) (100 fg, 10 pg, or 1 ng/mouse) significantly inhibited increases in skin thickness and wrinkle formation and the reduction in skin elasticity induced by long-term UVB irradiation. Furthermore, we examined the histological effects of total saponins and ginsenoside Rb(1) in the skin of UVB-irradiated hairless mice. The increases in apoptotic, Ki-67-, and 8-hydroxy-2'-deoxyguanosine-positive cells induced by UVB exposure were prevented by the topical application of total saponins and ginsenoside Rb(1). Furthermore, total saponins and ginsenoside Rb(1) prevented the disruption of collagen fibers induced by the long-term UVB irradiation. Ginsenoside Rb(1) (100 fg, 10 pg, and 1 ng/ml) increased the Bcl-2 expression level in UVB-treated human keratinocytes. The protective effect of ginsenoside Rb(1) on UVB-mediated apoptosis may be due to the up-regulation of Bcl-2 expression. These results suggest that the protective effect of ginsenoside Rb(1) on skin photoaging induced by chronic UVB exposure may be due to the increase in collagen synthesis and/or the inhibition of matrix metalloproteinase expression in dermal fibroblasts.
Authors:
Young Gon Kim; Maho Sumiyoshi; Masahiro Sakanaka; Yoshiyuki Kimura
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-19
Journal Detail:
Title:  European journal of pharmacology     Volume:  602     ISSN:  1879-0712     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-16     Completed Date:  2009-10-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  148-56     Citation Subset:  IM    
Affiliation:
Division of Functional Histology, Department of Functional Biomedicine, Ehime University Graduate School of Medicine, Shitsukawa, Toon City, Ehime 791-0295, Japan.
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MeSH Terms
Descriptor/Qualifier:
Administration, Topical
Animals
Apoptosis / drug effects,  physiology,  radiation effects
Cells, Cultured
Dose-Response Relationship, Drug
Fibroblasts / cytology,  drug effects,  radiation effects
Humans
Keratinocytes / cytology,  drug effects,  radiation effects
Male
Metalloendopeptidases / genetics,  metabolism
Mice
Mice, Hairless
Panax / chemistry*
Proto-Oncogene Proteins c-bcl-2 / genetics,  metabolism
Saponins / administration & dosage,  isolation & purification,  pharmacology*
Skin Aging / drug effects*,  radiation effects*
Ultraviolet Rays*
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins c-bcl-2; 0/Saponins; EC 3.4.24.-/Metalloendopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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