Document Detail


Effects of ginkgolides on interleukin-1, tumor necrosis factor-alpha and nitric oxide production by rat microglia stimulated with lipopolysaccharides in vitro.
MedLine Citation:
PMID:  9893925     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of ginkgolide A (CAS 15291-75-5, BN52020, GA) and B (CAS 15291-77-7, BN52021, GB) on interleukin (IL)-1, tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) production in resting and lipopolysaccharide (LPS)-stimulated neonatal rat microglia were studied. Apafant (CAS 105219-56-5), a platelet activating factor (PAF) antagonist of triazolobenzodiazepine type was used as control. The biological activities of IL-1 and TNF-alpha were tested by mouse thymocyte proliferation and L929 cytotoxicity assay, respectively. NO concentration was represented by nitrite and determined by Griess reaction. GA 1 nmol/1-10 mumol/l inhibited IL-1 production, and 100 nmol/l-10 mumol/l decreased TNF-alpha and NO production in dose-dependent manner. GB inhibited IL-1, TNF-alpha and NO production at the concentrations 10 nmol/l-10 mumol/l, 100 nmol/l-10 mumol/l and 10 nmol/l-10 mumol/l, respectively. Apafant inhibited IL-1, but not TNF-alpha and NO production. GB plus apafant (50 mumol/l) showed IL-1 and NO inhibitory effects, but not on TNF-alpha. The manner was different from that of GB or apafant alone. The results suggested that GA and GB inhibited proinflammatory cytokines and NO production from LPS-stimulated rat microglia, however, apafant inhibited IL-1 production only. The effects of GA and GB on proinflammatory cytokines and NO production from rat microglia do not seem to be based on PAF receptor antagonism. In addition, GA and GB are regarded as promising agents for the treatment of some neurodegenerative diseases in the central nervous system.
Authors:
Z Y Du; X Y Li
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Arzneimittel-Forschung     Volume:  48     ISSN:  0004-4172     ISO Abbreviation:  Arzneimittelforschung     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-02-18     Completed Date:  1999-02-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372660     Medline TA:  Arzneimittelforschung     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  1126-30     Citation Subset:  IM    
Affiliation:
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, The People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Azepines / pharmacology*
Cells, Cultured
Diterpenes*
Free Radical Scavengers / pharmacology*
Ginkgolides
Interleukin-1 / biosynthesis*
Lactones / pharmacology*
Lipopolysaccharides / pharmacology*
Microglia / drug effects,  metabolism*
Nitric Oxide / biosynthesis*
Platelet Aggregation Inhibitors / pharmacology*
Rats
Rats, Sprague-Dawley
Stimulation, Chemical
Triazoles / pharmacology*
Tumor Necrosis Factor-alpha / biosynthesis*
Chemical
Reg. No./Substance:
0/Azepines; 0/Diterpenes; 0/Free Radical Scavengers; 0/Ginkgolides; 0/Interleukin-1; 0/Lactones; 0/Lipopolysaccharides; 0/Platelet Aggregation Inhibitors; 0/Triazoles; 0/Tumor Necrosis Factor-alpha; 10102-43-9/Nitric Oxide; 105219-56-5/WEB 2086; 99796-69-7/ginkgolide B

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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