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Effects of gene‑activated matrix on autograft healing of anterior cruciate ligament.
MedLine Citation:
PMID:  23229157     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The aim of the present study was to observe the effects of gene‑activated matrix (GAM) on autograft healing of the anterior cruciate ligament. Forty‑eight rabbits were randomly divided into groups A and B. Rabbits were used to construct models of anterior cruciate ligament reconstruction. In group A, transforming growth factor (TGF)‑β1 was locally injected into the bone tunnel, while in group B, empty vector was administered. Tendons were removed to observe histology and ultrastructure and to evaluate biomechanics at postoperative months 1, 3 and 6. Optical microscopy revealed increased numbers of fibroblasts and collagen fibers in group A at each time‑point compared with B. Electron microscopy identified increased mitosis and abundance of fibroblasts, endoplasmic reticulum and mitochondria in group A at each time‑point compared with B. No significant difference was identified in the biomechanical parameters between the 2 groups at postoperative month 1. At postoperative months 3 and 6, maximum force and elastic modulus were greater in group A compared with group B (P<0.0.5). No significant differences in other biochemical parameters were observed at these time‑points. The healing ligament graft transfected with TGF‑β1 by GAM was observed to have improved tissue structure and biomechanical characteristics. The results of the current study may provide a theoretical basis for GAM application in ligament repair.
Authors:
Jian Qin; Zhi-Qi Hou; Hui Wang; Xin-Liang Wang; Yong-Yi Liu; Xing-Hua Gao; Yu-Jin Lin; Bao-Hua Huang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-30
Journal Detail:
Title:  Molecular medicine reports     Volume:  -     ISSN:  1791-3004     ISO Abbreviation:  Mol Med Report     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-12-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101475259     Medline TA:  Mol Med Report     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, P.R. China.
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