Document Detail

Effects of gemcitabine and gemcitabine in combination with carboplatin on five canine transitional cell carcinoma cell lines.
MedLine Citation:
PMID:  22849687     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To evaluate in vitro effects of gemcitabine alone and in combination with carboplatin on canine transitional cell carcinoma (TCC) cell lines.
SAMPLE: In vitro cultures of 5 canine TCC cell lines.
PROCEDURES: Cells were treated with gemcitabine, carboplatin, or a combination of both at various concentrations. Cell proliferation was assessed via a fluorescence-based microplate cell proliferation assay. Cell cycle was evaluated via propidium iodide staining, and apoptosis was assessed by measurement of caspase 3 and 7 enzymatic activity. Synergy between gemcitabine and carboplatin was quantified via combination index analyses.
RESULTS: Treatment of 5 canine TCC cell lines with gemcitabine or carboplatin decreased cell proliferation, increased apoptosis, and induced cell cycle arrest. Cell cycle arrest and apoptosis were markedly increased when cell lines were treated with both gemcitabine and carboplatin simultaneously or sequentially. Order of administration during sequential treatment did not consistently affect cell proliferation results in TCC cell lines. When TCC cell lines were treated with gemcitabine and carboplatin in combination at therapeutically relevant concentrations (gemcitabine concentration, < 10μM; carboplatin concentration, < 250μM), a significant decrease in cell proliferation was observed, compared with cell proliferation following treatment with gemcitabine or carboplatin alone. In combination, the effects of gemcitabine and carboplatin were synergistic in 3 of 5 cell lines and additive in the other 2.
CONCLUSIONS AND CLINICAL RELEVANCE: Gemcitabine had antitumor effects on canine TCC cells in vitro, and the combination of gemcitabine and carboplatin had synergistic activity at biologically achievable concentrations.
Joao Felipe de Brito Galvao; William C Kisseberth; Sridhar Murahari; Saikaew Sutayatram; Dennis J Chew; Nongnuch Inpanbutr
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of veterinary research     Volume:  73     ISSN:  1943-5681     ISO Abbreviation:  Am. J. Vet. Res.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-01     Completed Date:  2012-11-28     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  0375011     Medline TA:  Am J Vet Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1262-72     Citation Subset:  IM    
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA.
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MeSH Terms
Antimetabolites, Antineoplastic / therapeutic use*
Antineoplastic Agents / therapeutic use*
Carboplatin / therapeutic use
Carcinoma, Transitional Cell / drug therapy*
Cell Cycle
Cell Line, Tumor
Cell Proliferation
Deoxycytidine / analogs & derivatives*,  therapeutic use
Dog Diseases / drug therapy*
Drug Synergism
Drug Therapy, Combination / veterinary
Reg. No./Substance:
0/Antimetabolites, Antineoplastic; 0/Antineoplastic Agents; 41575-94-4/Carboplatin; 951-77-9/Deoxycytidine; B76N6SBZ8R/gemcitabine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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