Document Detail


Effects of gamma-alumina and hydroxyapatite coatings on the growth and metabolism of human osteoblasts.
MedLine Citation:
PMID:  8582908     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The behavior of human osteoblasts cultivated on hydroxyapatite or alumina-coated disks of Ti6AL4V was studied in vitro. Cell anchorage and spreading were observed by scanning electron microscopy. Cell growth was monitored by counting cells and measuring DNA at 5 h and 2,5, and 10 days after cell seeding. Cells grown for 10 days were labeled with 14C-proline and total protein and collagen synthesis were measured; the type of collagen was also determined. Both ceramics showed excellent biocompatibility. At 10 days of culture the cells showed a higher rate of proliferation on alumina than on hydroxyapatite. Neither ceramic altered the collagen biosynthesis or the osteoblast-like properties of the cells, as indicated by the high percentage of type I collagen.
Authors:
B Labat; A Chamson; J Frey
Related Documents :
21254968 - Glycosylation status of the c. albicans cell wall affects the efficiency of neutrophil ...
18689918 - Reinforcement of porous alginate scaffolds by incorporating electrospun fibres.
17997178 - Smart thermoresponsive coatings and surfaces for tissue engineering: switching cell-mat...
9741148 - Detection of the ki-67 proliferation associated nuclear epitope in normal canine tissue...
12054588 - Isolation of chinese hamster ovary cell pex mutants: two pex7-defective mutants.
18493058 - Ccr4 alters cell size in yeast by modulating the timing of cln1 and cln2 expression.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical materials research     Volume:  29     ISSN:  0021-9304     ISO Abbreviation:  J. Biomed. Mater. Res.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1996-03-19     Completed Date:  1996-03-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0112726     Medline TA:  J Biomed Mater Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1397-401     Citation Subset:  IM    
Affiliation:
Laboratoire de Biochimie, Faculté de médecine, Saint Etienne, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Aluminum Oxide / pharmacology*
Biocompatible Materials
Cell Division / drug effects
Cells, Cultured
Collagen / biosynthesis
DNA / biosynthesis
Humans
Hydroxyapatites / pharmacology*
Hydroxyproline / metabolism
Kinetics
Microscopy, Electron, Scanning
Osteoblasts / drug effects*,  metabolism
Proline / metabolism
Protein Biosynthesis
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Hydroxyapatites; 1344-28-1/Aluminum Oxide; 147-85-3/Proline; 51-35-4/Hydroxyproline; 9007-34-5/Collagen; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A study of reversibly inactivated lipases for use in a morphine-triggered naltrexone delivery system...
Next Document:  X-ray powder diffraction patterns of calcium phosphates analyzed by the Rietveld method.