Document Detail

Effects of gamma-alumina and hydroxyapatite coatings on the growth and metabolism of human osteoblasts.
MedLine Citation:
PMID:  8582908     Owner:  NLM     Status:  MEDLINE    
The behavior of human osteoblasts cultivated on hydroxyapatite or alumina-coated disks of Ti6AL4V was studied in vitro. Cell anchorage and spreading were observed by scanning electron microscopy. Cell growth was monitored by counting cells and measuring DNA at 5 h and 2,5, and 10 days after cell seeding. Cells grown for 10 days were labeled with 14C-proline and total protein and collagen synthesis were measured; the type of collagen was also determined. Both ceramics showed excellent biocompatibility. At 10 days of culture the cells showed a higher rate of proliferation on alumina than on hydroxyapatite. Neither ceramic altered the collagen biosynthesis or the osteoblast-like properties of the cells, as indicated by the high percentage of type I collagen.
B Labat; A Chamson; J Frey
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical materials research     Volume:  29     ISSN:  0021-9304     ISO Abbreviation:  J. Biomed. Mater. Res.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1996-03-19     Completed Date:  1996-03-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0112726     Medline TA:  J Biomed Mater Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1397-401     Citation Subset:  IM    
Laboratoire de Biochimie, Faculté de médecine, Saint Etienne, France.
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MeSH Terms
Aluminum Oxide / pharmacology*
Biocompatible Materials
Cell Division / drug effects
Cells, Cultured
Collagen / biosynthesis
DNA / biosynthesis
Hydroxyapatites / pharmacology*
Hydroxyproline / metabolism
Microscopy, Electron, Scanning
Osteoblasts / drug effects*,  metabolism
Proline / metabolism
Protein Biosynthesis
Reg. No./Substance:
0/Biocompatible Materials; 0/Hydroxyapatites; 1344-28-1/Aluminum Oxide; 147-85-3/Proline; 51-35-4/Hydroxyproline; 9007-34-5/Collagen; 9007-49-2/DNA

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