Document Detail

Effects of galanin-like peptide on food intake and the hypothalamo-pituitary-thyroid axis.
MedLine Citation:
PMID:  12624534     Owner:  NLM     Status:  MEDLINE    
Galanin-like peptide (GALP) is a novel hypothalamic peptide synthesised in neurons in the arcuate nucleus which project to the paraventricular nucleus (PVN). GALP has recently been identified as an orexigenic peptide. In this study we aimed to further characterise the hypothalamic action of this peptide in energy homeostasis. Firstly, we investigated the orexigenic effect of GALP in the PVN and compared its effects with galanin and galanin 2-29. Secondly, we examined the effect of PVN administration of GALP and galanin on circulating thyroid-stimulating hormone (TSH). PVN administration of GALP significantly increased the food intake of satiated rats 1 h after administration at doses of 0.3, 1 and 3 nmol. In comparison with paraventricular administration of galanin, GALP was a more potent orexigen, whereas galanin 2-29, the relatively selective GAL R2 agonist, had no effect on food intake. Both GALP and galanin administration (1 nmol) into the PVN significantly decreased the level of circulating TSH. To investigate the mechanism of these effects, we examined the effect of GALP and galanin application on neuropeptide release from hypothalamic explants in vitro. GALP peptide (100 nM) stimulated the release of the orexigenic peptide neuropeptide Y from hypothalamic explants and decreased the release of the anorectic peptide cocaine-and-amphetamine-regulated transcript, whereas galanin (100 nM) peptide had no significant effect on the release of either peptide. Both GALP (100 nM) and galanin (100 nM) inhibited the release thyrotrophin-releasing hormone. These data suggest that in the PVN, GALP may play a role in energy homeostasis by stimulating food intake and suppressing TSH release.
Asha Seth; Sarah Stanley; Waljit Dhillo; Kevin Murphy; Mohammad Ghatei; Sephen Bloom
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroendocrinology     Volume:  77     ISSN:  0028-3835     ISO Abbreviation:  Neuroendocrinology     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-03-07     Completed Date:  2003-04-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0035665     Medline TA:  Neuroendocrinology     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  125-31     Citation Subset:  IM    
Copyright Information:
Copyright 2003 S. Karger AG, Basel
Division of Metabolic Medicine, Faculty of Medicine, Imperial College of Science Technology and Medicine, Hammersmith Campus, London, UK.
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MeSH Terms
Appetite Regulation / drug effects*
Eating / drug effects*
Energy Metabolism / drug effects*
Galanin-Like Peptide
Hypothalamo-Hypophyseal System / drug effects*
Nerve Tissue Proteins / administration & dosage,  pharmacology*
Organ Culture Techniques
Rats, Wistar
Thyroid Gland / drug effects*
Reg. No./Substance:
0/Galanin-Like Peptide; 0/Nerve Tissue Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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