| Effects of galactose on direct and indirect pathway estimates of hepatic glycogen synthesis. | |
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MedLine Citation:
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PMID: 20797446 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Hepatic glycogen is formed by direct and indirect pathways whose activities reflect altered nutrition or disease. Direct/indirect pathway measurements often involve test meals where ∼ 10% of carbohydrate is galactose, but its effects on direct/indirect pathway estimates are unknown. Therefore, direct/indirect pathway contributions in 24-h fasted rats given 2g/kg 100% glucose (GLU, n=6) or 90% glucose-10% galactose (GLU+GAL, n=6) were measured by [U-(13)C]glucose dilution and by position-5/position-2 glycogen enrichment (H5/H2) from 2H(2)O. For GLU+GAL, galactose glycogenesis was independently measured with [1-(13)C]galactose. Glycogenesis was equivalent in both groups but for GLU+GAL, 23 ± 4% of glycogen was derived from galactose. [U-(13)C]glucose reported a 30 ± 3% direct pathway contribution to glycogenesis for GLU but only 20 ± 3% for GLU+GAL (p=0.012 vs. GLU). H5/H2 yielded identical direct pathway estimates (32 ± 3% GLU, 29 ± 6% GLU+GAL). Thus, galactose glycogenesis was undetected by H5/H2 while [U-(13)C]glucose reported a reduced direct/indirect pathway ratio. With [1-(13)C]galactose also present, correct glycogenic source contributions were obtained. |
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Authors:
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Ana F Soares; Rui A Carvalho; Francisco J Veiga; John G Jones |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-24 |
Journal Detail:
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Title: Metabolic engineering Volume: 12 ISSN: 1096-7184 ISO Abbreviation: Metab. Eng. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9815657 Medline TA: Metab Eng Country: Belgium |
Other Details:
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Languages: eng Pagination: 552-60 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Life Sciences and Center for Neurosciences and Cell Biology, University of Coimbra, Rua dos Estudos, Apartado 3126, 3001-401 Coimbra, Portugal. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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