Document Detail

Effects of freezing-induced cell-fluid-matrix interactions on the cells and extracellular matrix of engineered tissues.
MedLine Citation:
PMID:  21549425     Owner:  NLM     Status:  MEDLINE    
The two most significant challenges for successful cryopreservation of engineered tissues (ETs) are preserving tissue functionality and controlling highly tissue-type dependent preservation outcomes. In order to address these challenges, freezing-induced cell-fluid-matrix interactions should be understood, which determine the post-thaw cell viability and extracellular matrix (ECM) microstructure. However, the current understanding of this tissue-level biophysical interaction is still limited. In this study, freezing-induced cell-fluid-matrix interactions and their impact on the cells and ECM microstructure of ETs were investigated using dermal equivalents as a model ET. The dermal equivalents were constructed by seeding human dermal fibroblasts in type I collagen matrices with varying cell seeding density and collagen concentration. While these dermal equivalents underwent an identical freeze/thaw condition, their spatiotemporal deformation during freezing, post-thaw ECM microstructure, and cellular level cryoresponse were characterized. The results showed that the extent and characteristics of freezing-induced deformation were significantly different among the experimental groups, and the ETs with denser ECM microstructure experienced a larger deformation. The magnitude of the deformation was well correlated to the post-thaw ECM structure, suggesting that the freezing-induced deformation is a good indicator of post-thaw ECM structure. A significant difference in the extent of cellular injury was also noted among the experimental groups, and it depended on the extent of freezing-induced deformation of the ETs and the initial cytoskeleton organization. These results suggest that the cells have been subjected to mechanical insult due to the freezing-induced deformation as well as thermal insult. These findings provide insight on tissue-type dependent cryopreservation outcomes, and can help to design and modify cryopreservation protocols for new types of tissues from a pre-developed cryopreservation protocol.
Ka Yaw Teo; Tenok O DeHoyos; J Craig Dutton; Frederick Grinnell; Bumsoo Han
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-05-05
Journal Detail:
Title:  Biomaterials     Volume:  32     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-05-30     Completed Date:  2011-09-19     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  5380-90     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
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MeSH Terms
Cell Count
Cell Line, Transformed
Cell Survival
Collagen Type I / chemistry
Cryopreservation / methods*
Cytoskeleton / pathology
Dermis / cytology
Extracellular Matrix / pathology*,  ultrastructure
Fibroblasts / cytology,  pathology*
Microscopy, Electron, Scanning
Microscopy, Fluorescence
Quantum Dots
Tissue Engineering / methods*
Tissue Scaffolds / chemistry
Grant Support
R01 EB008388/EB/NIBIB NIH HHS; R01 EB008388/EB/NIBIB NIH HHS; R01 EB008388-03/EB/NIBIB NIH HHS; R01 EB008388-04/EB/NIBIB NIH HHS; R01 EB008388-05/EB/NIBIB NIH HHS
Reg. No./Substance:
0/Collagen Type I

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