| Effects of freezing-induced cell-fluid-matrix interactions on the cells and extracellular matrix of engineered tissues. | |
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MedLine Citation:
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PMID: 21549425 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The two most significant challenges for successful cryopreservation of engineered tissues (ETs) are preserving tissue functionality and controlling highly tissue-type dependent preservation outcomes. In order to address these challenges, freezing-induced cell-fluid-matrix interactions should be understood, which determine the post-thaw cell viability and extracellular matrix (ECM) microstructure. However, the current understanding of this tissue-level biophysical interaction is still limited. In this study, freezing-induced cell-fluid-matrix interactions and their impact on the cells and ECM microstructure of ETs were investigated using dermal equivalents as a model ET. The dermal equivalents were constructed by seeding human dermal fibroblasts in type I collagen matrices with varying cell seeding density and collagen concentration. While these dermal equivalents underwent an identical freeze/thaw condition, their spatiotemporal deformation during freezing, post-thaw ECM microstructure, and cellular level cryoresponse were characterized. The results showed that the extent and characteristics of freezing-induced deformation were significantly different among the experimental groups, and the ETs with denser ECM microstructure experienced a larger deformation. The magnitude of the deformation was well correlated to the post-thaw ECM structure, suggesting that the freezing-induced deformation is a good indicator of post-thaw ECM structure. A significant difference in the extent of cellular injury was also noted among the experimental groups, and it depended on the extent of freezing-induced deformation of the ETs and the initial cytoskeleton organization. These results suggest that the cells have been subjected to mechanical insult due to the freezing-induced deformation as well as thermal insult. These findings provide insight on tissue-type dependent cryopreservation outcomes, and can help to design and modify cryopreservation protocols for new types of tissues from a pre-developed cryopreservation protocol. |
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Authors:
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Ka Yaw Teo; Tenok O DeHoyos; J Craig Dutton; Frederick Grinnell; Bumsoo Han |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2011-05-05 |
Journal Detail:
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Title: Biomaterials Volume: 32 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-05-30 Completed Date: 2011-09-19 Revised Date: 2012-09-25 |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 5380-90 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
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School of Mechanical Engineering, Purdue University, West Lafayette, IN, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Count Cell Line, Transformed Cell Survival Collagen Type I / chemistry Cryopreservation / methods* Cytoskeleton / pathology Dermis / cytology Extracellular Matrix / pathology*, ultrastructure Fibroblasts / cytology, pathology* Freezing* Humans Microscopy, Electron, Scanning Microscopy, Fluorescence Quantum Dots Tissue Engineering / methods* Tissue Scaffolds / chemistry |
| Grant Support | |
ID/Acronym/Agency:
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R01 EB008388/EB/NIBIB NIH HHS; R01 EB008388-03/EB/NIBIB NIH HHS; R01 EB008388-04/EB/NIBIB NIH HHS; R01 EB008388-05/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Collagen Type I |
| Comments/Corrections | |
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