Document Detail

Effects of four different extenders and three different freezing rates on post-thaw viability of dog semen.
MedLine Citation:
PMID:  8229939     Owner:  NLM     Status:  MEDLINE    
Ejaculates were collected from 17 dogs. After initial evaluation the ejaculates were split into four equal aliquots and diluted with either Triladyl, Pipes, IMV Universal or Tris-fructose-citric acid extender, all containing egg yolk and glycerol. The extended semen was frozen in 0.5 ml straws at a slow, intermediate or fast freezing rate. After thawing, the percentage of progressively motile spermatozoa and the velocity of forward motion was evaluated. There was no difference between the extenders with regard to progressive motility. The slow, intermediate and fast freezing rates resulted in the highest, intermediate and lowest motility values, respectively, regardless of extender. All four extenders were equally suitable for freezing canine semen at a slow freezing rate.
I Dobrinski; C Lulai; A D Barth; K Post
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of reproduction and fertility. Supplement     Volume:  47     ISSN:  0449-3087     ISO Abbreviation:  J. Reprod. Fertil. Suppl.     Publication Date:  1993  
Date Detail:
Created Date:  1993-12-21     Completed Date:  1993-12-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0225652     Medline TA:  J Reprod Fertil Suppl     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  291-6     Citation Subset:  IM    
Department of Herd Medicine and Theriogenology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
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MeSH Terms
Cell Survival / physiology*
Cryopreservation / methods,  veterinary*
Cryoprotective Agents*
Sperm Motility / physiology
Spermatozoa / physiology
Time Factors
Reg. No./Substance:
0/Cryoprotective Agents

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