| Effects of food availability and administration of orexigenic and anorectic agents on elevated ethanol drinking associated with drinking in the dark procedures. | |
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MedLine Citation:
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PMID: 18782340 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Drinking in the dark (DID) procedures have recently been developed to induce high levels of ethanol drinking in C57BL/6J mice, which result in blood ethanol concentrations reaching levels that have measurable affects on physiology and/or behavior. The present study determined if increased ethanol drinking associated with DID procedures may be motivated by caloric need rather than by the postingestive pharmacological effects of ethanol. To this end, food availability was manipulated or mice were given peripheral administration of orexigenic or anorectic agents during DID procedures. METHODS: C57BL/6J had 2-hours of access to the 20% (v/v) ethanol solution beginning 3-hours into the dark cycle on days 1 to 3, and 4-hours of access to the ethanol bottle on day 4 of DID procedures. In Experiment 1, the effects of food deprivation on ethanol consumption during DID procedures was assessed. In Experiments 2 and 3, mice were given intraperitoneal (i.p.) injection of the orexigenic peptide ghrelin (0, 10 or 30 mg/kg) or the anorectic protein leptin (0 or 20 microg/g), respectively, before access to ethanol on day 4 of DID procedures. In Experiment 4, hourly consumption of food and a 0.05% saccharin solution were assessed over a period of hours that included those used with DID procedures. RESULTS: Consistent with previous research, mice achieved blood ethanol concentrations (BECs) that ranged between 100 and 150 mg% on day 4 of DID experiments. Neither food deprivation nor administration of orexigenic or anorectic compounds significantly altered ethanol drinking with DID procedures. Interestingly, mice exhibited their highest level of food and saccharin solution consumption during hours that overlapped with DID procedures. CONCLUSIONS: The present observations are inconsistent with the hypothesis that C57BL/6J mice consume large amounts of ethanol during DID procedures in order to satisfy a caloric need. |
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Authors:
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Angela M Lyons; Emily G Lowery; Dennis R Sparta; Todd E Thiele |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Alcoholism, clinical and experimental research Volume: 32 ISSN: 1530-0277 ISO Abbreviation: Alcohol. Clin. Exp. Res. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-11-26 Completed Date: 2009-02-09 Revised Date: 2011-02-02 |
Medline Journal Info:
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Nlm Unique ID: 7707242 Medline TA: Alcohol Clin Exp Res Country: England |
Other Details:
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Languages: eng Pagination: 1962-8 Citation Subset: IM |
Affiliation:
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Department of Psychology, Bowles Center for Alcohol Studies, University of North Carolina, Chapel Hill, North Carolina 27599-3270, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alcohol Drinking
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physiopathology* Alcoholism / physiopathology Animals Appetite Depressants / administration & dosage, pharmacology* Appetite Stimulants / administration & dosage, pharmacology* Darkness Disease Models, Animal Energy Intake / drug effects, physiology Ethanol / blood Feeding Behavior / drug effects Food Deprivation / physiology* Ghrelin / administration & dosage, pharmacology Injections, Intraperitoneal Leptin / administration & dosage, pharmacology Male Mice Mice, Inbred C57BL Saccharin / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AA013573/AA/NIAAA NIH HHS; AA015148/AA/NIAAA NIH HHS; AA015875/AA/NIAAA NIH HHS; R01 AA013573-01/AA/NIAAA NIH HHS; R01 AA015148-06/AA/NIAAA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Appetite Depressants; 0/Appetite Stimulants; 0/Ghrelin; 0/Leptin; 64-17-5/Ethanol; 81-07-2/Saccharin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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