Document Detail


Effects of food availability and administration of orexigenic and anorectic agents on elevated ethanol drinking associated with drinking in the dark procedures.
MedLine Citation:
PMID:  18782340     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Drinking in the dark (DID) procedures have recently been developed to induce high levels of ethanol drinking in C57BL/6J mice, which result in blood ethanol concentrations reaching levels that have measurable affects on physiology and/or behavior. The present study determined if increased ethanol drinking associated with DID procedures may be motivated by caloric need rather than by the postingestive pharmacological effects of ethanol. To this end, food availability was manipulated or mice were given peripheral administration of orexigenic or anorectic agents during DID procedures.
METHODS: C57BL/6J had 2-hours of access to the 20% (v/v) ethanol solution beginning 3-hours into the dark cycle on days 1 to 3, and 4-hours of access to the ethanol bottle on day 4 of DID procedures. In Experiment 1, the effects of food deprivation on ethanol consumption during DID procedures was assessed. In Experiments 2 and 3, mice were given intraperitoneal (i.p.) injection of the orexigenic peptide ghrelin (0, 10 or 30 mg/kg) or the anorectic protein leptin (0 or 20 microg/g), respectively, before access to ethanol on day 4 of DID procedures. In Experiment 4, hourly consumption of food and a 0.05% saccharin solution were assessed over a period of hours that included those used with DID procedures.
RESULTS: Consistent with previous research, mice achieved blood ethanol concentrations (BECs) that ranged between 100 and 150 mg% on day 4 of DID experiments. Neither food deprivation nor administration of orexigenic or anorectic compounds significantly altered ethanol drinking with DID procedures. Interestingly, mice exhibited their highest level of food and saccharin solution consumption during hours that overlapped with DID procedures.
CONCLUSIONS: The present observations are inconsistent with the hypothesis that C57BL/6J mice consume large amounts of ethanol during DID procedures in order to satisfy a caloric need.
Authors:
Angela M Lyons; Emily G Lowery; Dennis R Sparta; Todd E Thiele
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Alcoholism, clinical and experimental research     Volume:  32     ISSN:  1530-0277     ISO Abbreviation:  Alcohol. Clin. Exp. Res.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-26     Completed Date:  2009-02-09     Revised Date:  2011-02-02    
Medline Journal Info:
Nlm Unique ID:  7707242     Medline TA:  Alcohol Clin Exp Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  1962-8     Citation Subset:  IM    
Affiliation:
Department of Psychology, Bowles Center for Alcohol Studies, University of North Carolina, Chapel Hill, North Carolina 27599-3270, USA.
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MeSH Terms
Descriptor/Qualifier:
Alcohol Drinking / physiopathology*
Alcoholism / physiopathology
Animals
Appetite Depressants / administration & dosage,  pharmacology*
Appetite Stimulants / administration & dosage,  pharmacology*
Darkness
Disease Models, Animal
Energy Intake / drug effects,  physiology
Ethanol / blood
Feeding Behavior / drug effects
Food Deprivation / physiology*
Ghrelin / administration & dosage,  pharmacology
Injections, Intraperitoneal
Leptin / administration & dosage,  pharmacology
Male
Mice
Mice, Inbred C57BL
Saccharin / metabolism
Grant Support
ID/Acronym/Agency:
AA013573/AA/NIAAA NIH HHS; AA015148/AA/NIAAA NIH HHS; AA015875/AA/NIAAA NIH HHS; R01 AA013573-01/AA/NIAAA NIH HHS; R01 AA015148-06/AA/NIAAA NIH HHS
Chemical
Reg. No./Substance:
0/Appetite Depressants; 0/Appetite Stimulants; 0/Ghrelin; 0/Leptin; 64-17-5/Ethanol; 81-07-2/Saccharin
Comments/Corrections

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