| Effects of flow and contraction on lactate transport in the perfused rat hindlimb. | |
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MedLine Citation:
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PMID: 8048516 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A perfused rat hindlimb preparation was used to assess the effects of perfusate flow and electrical stimulation to mimic exercise on the rates of lactate influx (measured by a dual tracer technique with [3H]mannitol as the extracellular marker) and net lactate production. The same perfused muscle system was also used for assessing the effects of alpha-cyano-4-hydroxycinnamate (CIN, 15 mM), phloretin (0.6 mM), and pyruvate on tracer lactate influx. Unidirectional lactate influx, oxygen uptake (VO2), and net lactate flux were all significantly dependent on perfusate flow rate (all P < 0.05). The hindlimb was in net lactate production at all flow rates studied. Electrical stimulation (60 Hz, 100 ms, 20 V trains at 0.6 min-1) at perfusate lactate concentration of 1 mM significantly increased the hindlimb VO2 from 8.0 +/- 1.1 to 16.0 +/- 2.2 ml.kg-1.min-1 and production of lactate from -69 +/- 31 to -823 +/- 77 nmol.min-1.g-1 (both P < 0.001) but did not affect tracer-measured unidirectional lactate influx (nonstimulated: 235.4 +/- 78.1; stimulated: 235.0 +/- 31.0 nmol.min-1.g-1). At a perfusate flow of 0.55 ml.g-1.min-1 the unidirectional influx of 1 mM lactate was markedly inhibited (90 +/- 5%) by 15 mM CIN. CIN also significantly reduced VO2 from 6.2 +/- 0.16 to 4.45 +/- 0.57 ml.kg-1.min-1 (P < 0.05, n = 5). Phloretin (0.6 mM, n = 3) had no significant effect on lactate influx.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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P W Watt; L B Gladden; H S Hundal; R E Crawford |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 267 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1994 Jul |
Date Detail:
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Created Date: 1994-08-30 Completed Date: 1994-08-30 Revised Date: 2009-09-29 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: E7-13 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Physiology, University of Dundee, Scotland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport Coumaric Acids / pharmacology Electric Stimulation Female Lactates / metabolism* Lactic Acid Muscle Contraction* Muscles / metabolism*, physiology Perfusion Phloretin / pharmacology Pyruvates / pharmacology Pyruvic Acid Rats Rats, Wistar |
| Grant Support | |
ID/Acronym/Agency:
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1RO1AR-40342/AR/NIAMS NIH HHS; //Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Coumaric Acids; 0/Lactates; 0/Pyruvates; 127-17-3/Pyruvic Acid; 28166-41-8/alpha-cyano-4-hydroxycinnamate; 50-21-5/Lactic Acid; 60-82-2/Phloretin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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