| Effects of a fish oil-based emulsion on rat hepatoma cell invasion in culture. | |
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MedLine Citation:
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PMID: 17936197 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Total parenteral nutrition containing a lipid emulsion is often employed after surgical tumor resection. This study investigated the effects of a fish oil-based infusion on rat hepatoma cell invasion. METHODS: Rat ascites hepatoma cell line AH109A was precultured with a fish oil-based or safflower oil-based emulsion for 48 h. Changes in membranous fatty acid composition were evaluated by gas chromatography. The invasiveness of hepatoma cells was assessed by coculturing with mesentery-derived mesothelial cells. To examine ex vivo effects of the fish oil-based infusion on hepatoma invasion, sera were prepared from rats infused with fish oil- or safflower oil-based emulsion and the effects of these sera were assessed. To clarify the mechanism of inhibition of invasion by the fish oil-based emulsion, the effects of prostaglandin (PG) E(2) and PGE(3) on invasion were examined. RESULTS: Pretreatment with the fish oil-based emulsion reduced invasiveness without affecting growth compared with the safflower oil-based emulsion. Pretreatment with the sera from rats infused with the fish oil-based emulsion also reduced invasiveness compared with the sera from rats infused with the safflower oil-based emulsion. The addition of PGE(2) eliminated the inhibitory effect of the fish oil-based emulsion, and the addition of PGE(3) reduced the invasiveness of hepatoma cells pretreated with the safflower oil-based emulsion. CONCLUSION: These results suggest that the fish oil-based emulsion may have anti-invasive effects. Changes in the membranous fatty acid composition and consequent changes in the prostaglandins produced may be involved in this inhibitory effect. |
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Authors:
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Akifumi Hagi; Mitsuo Nakayama; Yutaka Miura; Kazumi Yagasaki |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nutrition (Burbank, Los Angeles County, Calif.) Volume: 23 ISSN: 0899-9007 ISO Abbreviation: Nutrition Publication Date: 2007 Nov-Dec |
Date Detail:
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Created Date: 2007-10-15 Completed Date: 2008-02-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8802712 Medline TA: Nutrition Country: United States |
Other Details:
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Languages: eng Pagination: 871-7 Citation Subset: IM |
Affiliation:
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Nutrition Research Institute, Otsuka Pharmaceutical Factory, Inc., Tokushima, Japan. hagiak@otsukakj.co.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alprostadil
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analogs & derivatives,
metabolism Animals Cell Division / drug effects* Cell Line, Tumor Cell Membrane / chemistry Dinoprostone / metabolism Fat Emulsions, Intravenous / pharmacology* Fatty Acids, Unsaturated / pharmacology Fish Oils / pharmacology* Liver Neoplasms, Experimental Male Neoplasm Invasiveness* Parenteral Nutrition, Total* Random Allocation Rats Safflower Oil / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Fat Emulsions, Intravenous; 0/Fatty Acids, Unsaturated; 0/Fish Oils; 363-24-6/Dinoprostone; 745-65-3/Alprostadil; 8001-23-8/Safflower Oil; 802-31-3/prostaglandin E3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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