Document Detail


Effects of fish-derived lipoprotein extracts on activation markers, Fas expression and apoptosis in peripheral blood lymphocytes.
MedLine Citation:
PMID:  15652756     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several factors may influence numbers and function of peripheral blood lymphocytes (PBLs) by different processes. We conducted this study to evaluate the effect of E-CAB-94011 and E-JUR-94013, two marine fish extracts from S. scombrus and T. trachurus, respectively, on in vitro PBLs activation and on the expression and functionality of Fas, a cell surface molecule that plays a central role in immune homeostasis and cytotoxic activity. PBLs from 24 healthy volunteers were isolated and flow cytometry was performed to measure the state of activation, Fas expression and apoptosis of PBLs. Functionality of Fas was tested by assessing apoptosis after incubation of isolated lymphocytes with agonistic anti-Fas antibodies in blood samples treated with both E-CAB-94011 and E-JUR-94013. Studies on the lymphocyte cell marker suggest a clear immune activation as measured by the increased levels of CD25, CD8, CD38, CD19 and HLA-DR in vitro expression on lymphocytes treated with both extracts. In addition, a significant reduction in the percentages of apoptotic CD19(+)CD38(+) double positive lymphocytes could be demonstrated in the treated samples with respect to controls (p<0.05). Therefore the present results indicate that both E-CAB-94011 and E-JUR-94013 in vitro are powerful immunoregulatory, increasing immune surveillance.
Authors:
V R M Lombardi; L Fernández-Novoa; I Etcheverría; S Seoane; R Cacabelos
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  International immunopharmacology     Volume:  5     ISSN:  1567-5769     ISO Abbreviation:  Int. Immunopharmacol.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-17     Completed Date:  2005-06-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100965259     Medline TA:  Int Immunopharmacol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  253-62     Citation Subset:  IM    
Affiliation:
EBIOTEC, Santa Marta de Babío s/n, EuroEspes Building 1st f., 15166 Bergondo, La Coruña, Spain. biotecnologia@ebiotec.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD95 / biosynthesis*
Apoptosis / drug effects*
Biological Products / pharmacology*
Cell Survival / drug effects
Complex Mixtures / pharmacology*
Fishes
Humans
Lipoproteins / pharmacology*
Lymphocyte Activation / drug effects*
Lymphocyte Subsets / drug effects,  metabolism
Lymphocytes / drug effects*,  metabolism
Tissue Extracts / pharmacology*
Chemical
Reg. No./Substance:
0/Antigens, CD95; 0/Biological Products; 0/Complex Mixtures; 0/Lipoproteins; 0/Tissue Extracts

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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