Document Detail


Effects of feeding bile acids and a bile acid sequestrant on hepatic bile acid composition in mice.
MedLine Citation:
PMID:  20671298     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An improved ultra performance liquid chromatography-tandem mass spectrometry (UPLC/MS/MS) method was established for the simultaneous analysis of various bile acids (BA) and applied to investigate liver BA content in C57BL/6 mice fed 1% cholic acid (CA), 0.3% deoxycholic acid (DCA), 0.3% chenodeoxycholic acid (CDCA), 0.3% lithocholic acid (LCA), 3% ursodeoxycholic acid (UDCA), or 2% cholestyramine (resin). Results indicate that mice have a remarkable ability to maintain liver BA concentrations. The BA profiles in mouse livers were similar between CA and DCA feedings, as well as between CDCA and LCA feedings. The mRNA expression of Cytochrome P450 7a1 (Cyp7a1) was suppressed by all BA feedings, whereas Cyp7b1 was suppressed only by CA and UDCA feedings. Gender differences in liver BA composition were observed after feeding CA, DCA, CDCA, and LCA, but they were not prominent after feeding UDCA. Sulfation of CA and CDCA was found at the 7-OH position, and it was increased by feeding CA or CDCA more in male than female mice. In contrast, sulfation of LCA and taurolithocholic acid (TLCA) was female-predominant, and it was increased by feeding UDCA and LCA. In summary, the present systematic study on BA metabolism in mice will aid in interpreting BA-mediated gene regulation and hepatotoxicity.
Authors:
Youcai Zhang; Curtis D Klaassen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-07-29
Journal Detail:
Title:  Journal of lipid research     Volume:  51     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-13     Completed Date:  2011-01-19     Revised Date:  2014-09-11    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3230-42     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bile Acids and Salts / chemistry,  isolation & purification,  metabolism*,  pharmacology*
Chenodeoxycholic Acid / metabolism
Chromatography, High Pressure Liquid
Dietary Supplements*
Female
Gene Expression Regulation / drug effects
Liver / drug effects*,  metabolism*
Male
Mice
Mice, Inbred C57BL
RNA, Messenger / genetics,  metabolism
Reproducibility of Results
Sex Factors
Sulfates / metabolism
Tandem Mass Spectrometry
Grant Support
ID/Acronym/Agency:
ES-009649/ES/NIEHS NIH HHS; ES-009716/ES/NIEHS NIH HHS; ES-013714/ES/NIEHS NIH HHS; P20 RR021940/RR/NCRR NIH HHS; R01 ES009649/ES/NIEHS NIH HHS; R01 ES009649-07/ES/NIEHS NIH HHS; R01 ES009649-08A2/ES/NIEHS NIH HHS; R01 ES009649-09/ES/NIEHS NIH HHS; R01 ES009649-10/ES/NIEHS NIH HHS; R01 ES009716/ES/NIEHS NIH HHS; R01 ES009716-08/ES/NIEHS NIH HHS; R01 ES009716-09/ES/NIEHS NIH HHS; R01 ES009716-10/ES/NIEHS NIH HHS; R01 ES013714/ES/NIEHS NIH HHS; R01 ES013714-03/ES/NIEHS NIH HHS; R01 ES013714-04/ES/NIEHS NIH HHS; R01 ES013714-05/ES/NIEHS NIH HHS; RR-021940/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Bile Acids and Salts; 0/RNA, Messenger; 0/Sulfates; 0GEI24LG0J/Chenodeoxycholic Acid
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