Document Detail

Effects of fatty acids on apolipoprotein B secretion by McArdle RH-7777 rat hepatoma cells.
MedLine Citation:
PMID:  9233686     Owner:  NLM     Status:  MEDLINE    
The effect of oleic acid (OA), stearic acid (SA) and elaidic acid (EA) on cellular and secreted apolipoprotein (apo) B was examined in McArdle RH-7777 (McArdle) hepatoma cells and in primary rat hepatocytes. ApoB secretion by McArdle cells was significantly inhibited by 20% in 8 h incubations in medium containing EA and SA and by 50% in medium containing OA. In contrast, apo B secretion and cellular apo B of primary rat hepatocytes was relatively unaffected by incubations in medium containing fatty acids. Both B100 and B48 secretion in McArdle wild type and B48 in apo B mRNA editing enzyme catalytic polypeptide transfectants expressing B48 were inhibited to a similar extent indicating an effect of OA on both apo B species. The effect of OA occurred without changes in cellular apo B or in apo B mRNA abundance suggesting a post-transcriptional mechanism. Time course studies indicate that the suppressive effect of OA requires 4 h of incubation suggesting the depletion of a limiting factor important in apoB secretion. By increasing the proportion of palmitic acid to OA in the medium, apoB secretion by McArdle cells was progressively restored to control levels implicating an unique role for newly synthesized saturated fatty acid.
J D Sparks; H L Collins; I Sabio; M P Sowden; H C Smith; J Cianci; C E Sparks
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1347     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-08-25     Completed Date:  1997-08-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  51-61     Citation Subset:  IM    
Department of Pathology and Laboratory Medicine, University of Rochester School of Medicine and Dentistry, NY 14642, USA.
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MeSH Terms
Apolipoproteins B / secretion*
Liver / metabolism*
Liver Neoplasms, Experimental / metabolism*
Oleic Acid / pharmacology*
Rats, Sprague-Dawley
Stearic Acids / pharmacology*
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Apolipoproteins B; 0/Stearic Acids; 112-79-8/elaidic acid; 112-80-1/Oleic Acid; 57-11-4/stearic acid

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