Document Detail

Effects of extraction condition on structural features and anticoagulant activity of F. vesca L. conjugates.
MedLine Citation:
PMID:  23218362     Owner:  NLM     Status:  In-Data-Review    
From the air-dried Wild strawberry (Fragaria vesca L., family Rosaceae) leaves five water-soluble glycoconjugates Fv I-V by different extraction conditions have been isolated. Effects of extraction steps/agents on chemical composition and anticoagulant activity of Fv I-V were examined. Dark brown F. vesca conjugates Fv I-V were recovered in 4.5-8.4% yields, based on dry herb. Isolates were composed of carbohydrate, phenolic and protein components. Fv I-V displayed on HPLC broad molecule-mass distribution patterns with dominance of low molecule-masses 9-14kDa. Their carbohydrate parts revealed high hexuronic acids content (35-60%) while the dominant neutral sugars - galactose, arabinose and rhamnose were found in lower amounts and indicated the presence of rhamnogalacturonans associated with arabinogalactans in all F. vesca preparations. In all Fv I-V isolates high polyphenolic contents were determined, whereas proteins were found in low amounts only. In in vitro experiments on human pooled plasma Fv I-V showed at higher concentrations complete inhibition of plasma clot formation and the most active conjugates in aPTT, PT and TT tests were shown to be Fv I and Fv III, containing the highest amounts of phenolics.
Izabela Pawlaczyk; Marta Lewik-Tsirigotis; Peter Capek; Mária Matulová; Vlasta Sasinková; Paweł Dąbrowski; Wojciech Witkiewicz; Roman Gancarz
Publication Detail:
Type:  Journal Article     Date:  2012-10-12
Journal Detail:
Title:  Carbohydrate polymers     Volume:  92     ISSN:  1879-1344     ISO Abbreviation:  Carbohydr Polym     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8307156     Medline TA:  Carbohydr Polym     Country:  England    
Other Details:
Languages:  eng     Pagination:  741-50     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Regional Specialist Hospital in Wrocław, Research and Development Centre, ul. Kamieńskiego 73a, 51-124 Wrocław, Poland; Organic and Pharmaceutical Technology Group, Chemistry Department, Wrocław University of Technology, Wybrzeże Wyspiańskiego 29, 50-370 Wrocław, Poland.
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