| Effects of extraction condition on structural features and anticoagulant activity of F. vesca L. conjugates. | |
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MedLine Citation:
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PMID: 23218362 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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From the air-dried Wild strawberry (Fragaria vesca L., family Rosaceae) leaves five water-soluble glycoconjugates Fv I-V by different extraction conditions have been isolated. Effects of extraction steps/agents on chemical composition and anticoagulant activity of Fv I-V were examined. Dark brown F. vesca conjugates Fv I-V were recovered in 4.5-8.4% yields, based on dry herb. Isolates were composed of carbohydrate, phenolic and protein components. Fv I-V displayed on HPLC broad molecule-mass distribution patterns with dominance of low molecule-masses 9-14kDa. Their carbohydrate parts revealed high hexuronic acids content (35-60%) while the dominant neutral sugars - galactose, arabinose and rhamnose were found in lower amounts and indicated the presence of rhamnogalacturonans associated with arabinogalactans in all F. vesca preparations. In all Fv I-V isolates high polyphenolic contents were determined, whereas proteins were found in low amounts only. In in vitro experiments on human pooled plasma Fv I-V showed at higher concentrations complete inhibition of plasma clot formation and the most active conjugates in aPTT, PT and TT tests were shown to be Fv I and Fv III, containing the highest amounts of phenolics. |
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Authors:
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Izabela Pawlaczyk; Marta Lewik-Tsirigotis; Peter Capek; Mária Matulová; Vlasta Sasinková; Paweł Dąbrowski; Wojciech Witkiewicz; Roman Gancarz |
Publication Detail:
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Type: Journal Article Date: 2012-10-12 |
Journal Detail:
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Title: Carbohydrate polymers Volume: 92 ISSN: 1879-1344 ISO Abbreviation: Carbohydr Polym Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2012-12-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8307156 Medline TA: Carbohydr Polym Country: England |
Other Details:
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Languages: eng Pagination: 741-50 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Elsevier Ltd. All rights reserved. |
Affiliation:
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Regional Specialist Hospital in Wrocław, Research and Development Centre, ul. Kamieńskiego 73a, 51-124 Wrocław, Poland; Organic and Pharmaceutical Technology Group, Chemistry Department, Wrocław University of Technology, Wybrzeże Wyspiańskiego 29, 50-370 Wrocław, Poland. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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