Document Detail

Effects of extracellular pressure and alcohol on the microglial response to inflammatory stimulation.
MedLine Citation:
PMID:  23140827     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Traumatic brain injury induces a neuroinflammatory response frequently associated with increased intracranial pressure. The aim of this study was to investigate the effects of alcohol and increased extracellular pressure on murine BV-2 microglial proliferation and cytokine responses to lipopolysaccharide (LPS) stimulation.
METHODS: BV-2 cells were cultured under 0 or 30 mm Hg increased extracellular pressure without or with ethanol (100 mmol/L) or LPS (10 ng/mL) for 24 hours. Cell proliferation was assessed using MTS assay and secretion of the proinflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-6, and monocyte chemotactic protein (MCP)-1 by enzyme-linked immunosorbent assay.
RESULTS: Increased pressure and LPS stimulation each promoted proliferation. Ethanol pretreatment blocked these effects. Basal TNF-α and IL-6 secretion was at the limits of delectability. Basal MCP-1 production was stimulated by pressure, which was blocked by ethanol. Even this low LPS dose stimulated microglial secretion of TNF-α, IL-6, and MCP-1. Pressure inhibited LPS-stimulated production of these proinflammatory cytokines, while ethanol pretreatment blocked LPS-stimulated cytokine production. The combination of pressure and ethanol further reduced TNF-α, IL-6, and MCP-1 secretion by LPS-stimulated microglial cells.
CONCLUSION: Alcohol's anti-inflammatory effects may contribute to the reduced mortality from traumatic brain injury that some have described in acutely intoxicated patients, while pressure down-regulation of inflammatory cytokine release could create a negative feedback that ameliorates inflammation in traumatic brain injury.
Lakshmi S Chaturvedi; Ping Zhang; Marc D Basson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  American journal of surgery     Volume:  204     ISSN:  1879-1883     ISO Abbreviation:  Am. J. Surg.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-12     Completed Date:  2013-01-15     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  0370473     Medline TA:  Am J Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  602-6     Citation Subset:  AIM; IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
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MeSH Terms
Biological Markers / metabolism
Cell Proliferation / drug effects
Cells, Cultured
Chemokine CCL2 / metabolism
Enzyme-Linked Immunosorbent Assay
Ethanol / pharmacology*
Interleukin-6 / metabolism
Lipopolysaccharides / pharmacology*
Microglia / drug effects*,  metabolism
Pressure / adverse effects*
Tumor Necrosis Factor-alpha / metabolism
Grant Support
Reg. No./Substance:
0/Biological Markers; 0/Chemokine CCL2; 0/Interleukin-6; 0/Lipopolysaccharides; 0/Tumor Necrosis Factor-alpha; 3K9958V90M/Ethanol

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