Document Detail


Effects of exopolysaccharide production on liquid vegetative growth, stress survival, and stationary phase recovery in Myxococcus xanthus.
MedLine Citation:
PMID:  22538652     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Exopolysaccharide (EPS) of Myxococcus xanthus is a well-regulated cell surface component. In addition to its known functions for social motility and fruiting body formation on solid surfaces, EPS has also been proposed to play a role in multi-cellular clumping in liquid medium, though this phenomenon has not been well studied. In this report, we confirmed that M. xanthus clumps formed in liquid were correlated with EPS levels and demonstrated that the EPS encased cell clumps exhibited biofilm-like structures. The clumps protected the cells at physiologically relevant EPS concentrations, while cells lacking EPS exhibited significant reduction in long-term viability and resistance to stressful conditions. However, excess EPS production was counterproductive to vegetative growth and viable cell recovery declined in extended late stationary phase as cells became trapped in the matrix of clumps. Therefore, optimal EPS production by M. xanthus is important for normal physiological functions in liquid.
Authors:
Wei Hu; Jing Wang; Ian McHardy; Renate Lux; Zhe Yang; Yuezhong Li; Wenyuan Shi
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-04-27
Journal Detail:
Title:  Journal of microbiology (Seoul, Korea)     Volume:  50     ISSN:  1976-3794     ISO Abbreviation:  J. Microbiol.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-27     Completed Date:  2012-08-13     Revised Date:  2013-12-06    
Medline Journal Info:
Nlm Unique ID:  9703165     Medline TA:  J Microbiol     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  241-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Culture Media / metabolism*
Extracellular Space / metabolism*
Microbial Viability
Myxococcus xanthus / growth & development*,  metabolism*
Polysaccharides, Bacterial / metabolism*
Grant Support
ID/Acronym/Agency:
GM54666/GM/NIGMS NIH HHS; R01 GM054666/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/Polysaccharides, Bacterial
Comments/Corrections

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