Document Detail

Effects of exogenous nitric oxide on oral squamous cell carcinoma: an in vitro study.
MedLine Citation:
PMID:  12149736     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Nitric oxide (NO) is a newly found unstable free radical gas, serving as an important mediator, messenger, and signal transduction molecule and involved in a variety of pathophysiologic processes. Recently, NO has been reported to have cytotoxic effects on several tumor cells as an effector molecule of activated macrophage. The objective of this study was to investigate the effects of exogenous NO on oral squamous cell carcinoma cell line and to try to clarify the possible mechanisms by which it kills tumor cells. METHODS: TSCCa cell line, established from a patient with oral squamous cell carcinoma of the tongue, was exposed to various concentrations of exogenous NO that were released from an NO donor, sodium nitroprusside (SNP), for 48 hours. Nitrite/nitrate levels in the culture supernatant were determined with a commercial available NO kit. Both morphologic and ultrastructural changes were evaluated by reverse phase contrast microscopy or transmission electron microscopy. The DNA was harvested from SNP-treated or untreated TSCCa cells and assessed by agarose gel electrophoresis. RESULTS: SNP released NO into medium in a dose-dependent manner. NO had a concentration-dependent cytotoxicity against TSCCa cells. NO induced tumor cell death through apoptosis, which was characterized by incompleteness of nuclear membrane, disappearance of nucleole and nuclear condensation, chromatin margination, or chromatin homogenization. Agarose gel electrophoresis showed a typical internucleosomal DNA cleavage pattern (DNA ladder), a reliable indicator of apoptosis. CONCLUSIONS: Our results suggested that NO had a tumoricidal potential against oral cancer cells. NO might exert its cytotoxicity as an effector molecule of activated microphage through at least apoptosis.
Zheng-Jun Shang; Jin-Rong Li; Zu-Bing Li
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons     Volume:  60     ISSN:  0278-2391     ISO Abbreviation:  J. Oral Maxillofac. Surg.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-07-31     Completed Date:  2002-08-30     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8206428     Medline TA:  J Oral Maxillofac Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  905-10; discussion 910-1     Citation Subset:  AIM; D; IM    
Copyright Information:
Copyright 2002 American Association of Oral and Maxillofacial Surgeons.
Department of Oral and Maxillofacial Surgery, College and Hospital of Stomatology, Wuhan University, Wuhan, People's Republic of China.
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MeSH Terms
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects
Carcinoma, Squamous Cell / pathology*,  ultrastructure
Cell Death / drug effects
Cell Nucleolus / drug effects,  ultrastructure
Cell Nucleus / drug effects,  ultrastructure
Chromatin / drug effects,  ultrastructure
DNA Fragmentation
DNA, Neoplasm / analysis
Dose-Response Relationship, Drug
Electrophoresis, Agar Gel
Free Radical Scavengers / pharmacology*
Microscopy, Electron
Microscopy, Phase-Contrast
Nitrates / analysis
Nitric Oxide / pharmacology*
Nitric Oxide Donors / administration & dosage,  pharmacology
Nitrites / analysis
Nitroprusside / administration & dosage,  pharmacology
Nuclear Envelope / drug effects,  ultrastructure
Nucleosomes / drug effects,  ultrastructure
Tongue Neoplasms / pathology*,  ultrastructure
Tumor Cells, Cultured
Reg. No./Substance:
0/Antineoplastic Agents; 0/Chromatin; 0/DNA, Neoplasm; 0/Free Radical Scavengers; 0/Nitrates; 0/Nitric Oxide Donors; 0/Nitrites; 0/Nucleosomes; 10102-43-9/Nitric Oxide; 15078-28-1/Nitroprusside
Comment In:
J Oral Maxillofac Surg. 2003 Feb;61(2):277   [PMID:  12619013 ]

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