Document Detail


Effects of exogenous GSH and methionine on methylation of inorganic arsenic in mice exposed to arsenite through drinking water.
MedLine Citation:
PMID:  19449390     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We hypothesized that chronic exposure to arsenic would deplete the reduced glutathione (GSH) and methionine in vivo, thereby impair the methylation capacity of inorganic arsenic (iAs) ingested. Our experiment was designed to explore the effects of exogenous GSH and methionine on arsenic methylation in mice exposed to arsenite via drinking water. Levels of iAs, monomethylarsenic acid (MMAs), and dimethylarsenic acid (DMAs) in the liver and blood were determined by the method of hydride generation coupled with atomic absorption spectrophotometry. Compared with mice exposed to arsenite alone, administration of GSH or methionine increased the secondary methylation index in the liver and primary methylation index in the blood, which resulted in the consequent increase of DMAs percent and decrease of iAs percent in the blood. Moreover, administration of GSH resulted in the increase of DMAs percent in the liver and total arsenic in the blood. Increase of total arsenic in the blood was mainly due to the increase of DMAs. Findings from the present study suggested that administration of GSH or methionine might potentiate the methylation capacity of arsenic in both liver and extrahepatic tissues, which may facilitate the excretion of arsenic and decrease arsenic related toxicities in the body.
Authors:
Yaping Jin; Fenghong Zhao; Yuan Zhong; Xiaoyun Yu; Di Sun; Yingjun Liao; Xiuqiang Lv; Gexin Li; Guifan Sun
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Environmental toxicology     Volume:  25     ISSN:  1522-7278     ISO Abbreviation:  Environ. Toxicol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-19     Completed Date:  2011-01-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100885357     Medline TA:  Environ Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  361-6     Citation Subset:  IM    
Affiliation:
Department of Environmental and Occupational Health, School of Public Health, China Medical University, Shenyang, Liaoning, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arsenic / blood,  toxicity*
Arsenic Poisoning / metabolism
Arsenites / toxicity*
Cacodylic Acid / metabolism
Drinking
Environmental Exposure*
Female
Glutathione / metabolism*,  pharmacology
Liver / metabolism
Methionine / metabolism*,  pharmacology
Methylation
Mice
Mice, Inbred Strains
Chemical
Reg. No./Substance:
0/Arsenites; 15502-74-6/arsenite; 63-68-3/Methionine; 70-18-8/Glutathione; 7440-38-2/Arsenic; 75-60-5/Cacodylic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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