Document Detail


Effects of exercise training on vasodilatory protein expression and activity in rats.
MedLine Citation:
PMID:  20680328     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Increased endothelium-dependent vasodilatation is associated with endurance exercise training. The purpose of this study was to test the hypothesis that increased endothelial nitric oxide synthase (eNOS) protein function, but not increased vascular smooth muscle sensitivity to NO, underlies augmented endothelium-dependent dilatation with training. To test these hypotheses, rats ran on a treadmill at 30 m/min (10% grade) for 60 min/day, 5 days/week, over 8-12 weeks (Trn). Training efficacy was demonstrated by greater (P < 0.05) hindlimb muscle citrate synthase activity and left ventricular mass-body mass ratio in Trn compared with sedentary control rats (Sed). Expression of eNOS protein in the aorta was increased with training (Sed, 1.00 ± 0.18 normalized units; Trn, 1.55 ± 0.23; P < 0.05). Aortic NOS activity was, however, unchanged by training (Sed, 1,505 ± 288 fmol/h/mg protein; Trn, 1,650 ± 247; n.s.). Expression of heat shock protein 90 and protein kinase B/Akt was not different between groups, nor was their association with eNOS. In follow-up series of rats, phosphorylated eNOS content (Serine 1177) was similar for Sed and Trn in both the aorta and gastrocnemius feed artery. Aortic NOS activity with eNOS phosphorylation status preserved was also similar between groups. Finally, cGMP concentration with a NO donor did not differ between groups (Sed, 73.0 ± 20.2 pmol/mg protein; Trn, 62.5 ± 12.9; n.s.). These findings indicate that training-induced increases in eNOS protein expression are not coupled to augmented function, illustrating the complexity of eNOS regulation. Further, they show that vascular sensitivity to NO is not altered by exercise training.
Authors:
Richard M McAllister; Elmer M Price
Related Documents :
8929618 - Exercise increases the plasma membrane content of the na+ -k+ pump and its mrna in rat ...
17118658 - Poloxamer 188 failed to prevent exercise-induced membrane breakdown in mdx skeletal mus...
11299288 - Intracellular signaling specificity in skeletal muscle in response to different modes o...
3693188 - Initiating regular exercise protects against muscle atrophy from glucocorticoids.
8027968 - Changes in selected parameters of health and physical fitness following a sixteen-week ...
24820738 - Dyspnea in eisenmenger syndrome and its amelioration by sildenafil: role of j receptors.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-08-03
Journal Detail:
Title:  European journal of applied physiology     Volume:  110     ISSN:  1439-6327     ISO Abbreviation:  Eur. J. Appl. Physiol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100954790     Medline TA:  Eur J Appl Physiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1019-27     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Physiology, Kansas State University, Manhattan, KS 66506, USA. mcallisterr@missouri.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
HL 52490/HL/NHLBI NIH HHS; HL 57226/HL/NHLBI NIH HHS; RR 18276/RR/NCRR NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Reply to Nolte and Noakes' "comments on Baker et al.'s change in body mass accurately and reliably p...
Next Document:  The effect of sealing with a fixed combination of collagen matrix-bound coagulation factors on the h...