Document Detail


Effects of exercise training on insulin-regulatable glucose-transporter protein levels in rat skeletal muscle.
MedLine Citation:
PMID:  2227115     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Exercise training has been shown to enhance the ability of insulin to stimulate glucose uptake in responsive tissues. The purpose of this study was to determine the effects of exercise training on the levels of the insulin-regulatable glucose transporter (IRGT) in rat skeletal muscle. After 6 wk of voluntary running in exercise-wheel cages, male Sprague-Dawley rats were rested for approximately 27 h and fasted overnight before removal of plantaris and soleus muscles. The concentration of glucose transporters per unit of muscle protein or DNA was quantitated by immunoblotting with an anti-IRGT polyclonal antibody raised against a synthetic peptide. The IRGT protein was increased by 60% (141 +/- 14 vs. 229 +/- 24 counts/min [cpm]/25 micrograms protein, P less than 0.01) in plantaris muscle from exercise-trained rats compared with controls. Total protein yield, DNA content, and 5'-nucleotidase activity were not different in plantaris muscle from control and exercise-trained rats. In contrast, there was no significant increase in the IRGT protein in soleus muscle after training when data were expressed per unit of muscle protein (292 +/- 22 vs. 346 +/- 16 cpm/25 micrograms protein). These data indicate that the increase in the IRGT in plantaris muscle is a selective response to exercise training that does not reflect an overall increase in muscle protein. The changes in IRGT for these muscles with exercise training parallel changes observed in insulin-mediated glucose uptake. We propose that this increase in the total number of glucose transporters may be a major component of the increase in insulin-mediated glucose uptake that is observed with exercise training.
Authors:
K J Rodnick; J O Holloszy; C E Mondon; D E James
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Diabetes     Volume:  39     ISSN:  0012-1797     ISO Abbreviation:  Diabetes     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1990-12-04     Completed Date:  1990-12-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372763     Medline TA:  Diabetes     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1425-9     Citation Subset:  AIM; IM; S    
Affiliation:
Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / immunology
Biological Transport / physiology
DNA / analysis
Glucose / pharmacokinetics
Immunoblotting
Insulin / physiology*
Male
Monosaccharide Transport Proteins / analysis,  immunology,  metabolism*
Muscles / chemistry,  metabolism*
Physical Conditioning, Animal*
Rats
Rats, Inbred Strains
Grant Support
ID/Acronym/Agency:
AG-00078/AG/NIA NIH HHS; DK-18986/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies; 0/Monosaccharide Transport Proteins; 11061-68-0/Insulin; 50-99-7/Glucose; 9007-49-2/DNA

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