Document Detail

Effects of exercise and steroid on skeletal muscle apoptosis in the mdx mouse.
MedLine Citation:
PMID:  15372534     Owner:  NLM     Status:  MEDLINE    
Reports concerning the influence of exercise loading and steroid administration on dystrophinopathy are inconsistent. To investigate the effect of muscle exercise in Duchenne muscular dystrophy (DMD), 15 control and 15 mdx mice, an animal model of DMD, were divided into free-living (n = 6), exercise (n = 6), and immobilization (n = 3) groups. Free-living and exercise groups were further divided into steroid-treated and sham-treated groups to evaluate the effect of steroid administration. We measured apoptotic changes by in situ DNA nick-end labeling (TUNEL), DNA fragmentation assay, and Western blotting for Bcl-2 and BAX. Apoptosis was most prominent in the sham-treated exercise group, and it was significantly reduced in the steroid-treated exercise group. The steroid-treated free-living group showed a higher rate of apoptotic change than the sham-treated free-living group. Apoptosis was minimized in the free-living condition, whereas exercise loading and immobilization caused apoptotic change in this muscular dystrophy animal model. Steroid administration induced apoptosis in muscle of free-living mice, but alleviated the apoptotic damage caused by exercise loading in mdx mice. These findings suggest that steroid administration may be effective in preventing a postexercise deterioration of skeletal muscle in animal models of DMD.
Jeong-Hoon Lim; Dai-Youl Kim; Moon Suk Bang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Muscle & nerve     Volume:  30     ISSN:  0148-639X     ISO Abbreviation:  Muscle Nerve     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-09-21     Completed Date:  2004-10-26     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7803146     Medline TA:  Muscle Nerve     Country:  United States    
Other Details:
Languages:  eng     Pagination:  456-62     Citation Subset:  IM    
Department of Rehabilitation Medicine, Seoul National University College of Medicine, 28 Yongon-dong Chongno-gu, 110-744, Republic of Korea.
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MeSH Terms
Apoptosis / drug effects*
Blotting, Western
Chromosomes / chemistry,  genetics
Cyclin D1 / metabolism
DNA / chemistry,  genetics
DNA Fragmentation
Electrophoresis, Polyacrylamide Gel
In Situ Nick-End Labeling
Mice, Inbred C57BL
Mice, Inbred mdx
Muscle, Skeletal / cytology*,  drug effects
Physical Exertion / physiology*
Proto-Oncogene Proteins / metabolism
Proto-Oncogene Proteins c-bcl-2*
Steroids / pharmacology*
bcl-2-Associated X Protein
Reg. No./Substance:
0/Bax protein, mouse; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/Steroids; 0/bcl-2-Associated X Protein; 136601-57-5/Cyclin D1; 9007-49-2/DNA

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