Document Detail


Effects of estradiol and progesterone on body composition, protein synthesis, and lipoprotein lipase in rats.
MedLine Citation:
PMID:  11171605     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Prior studies suggest that estradiol and progesterone regulate body composition in growing female rats. Because these studies did not consider the confounding effect of changes in food intake, it remains unclear whether ovarian hormones regulate body composition independently of their effects on food intake. We utilized a pair-feeding paradigm to examine the effects of these hormones on body composition. In addition, skeletal muscle protein fractional synthesis rate and adipose tissue lipoprotein lipase activity were measured to examine pathways of substrate deposition into fat and fat-free tissue. Female Sprague-Dawley rats [pubertal: 7-8 wk old; 190 +/- 0.5 (SE) g] were separated into four groups: 1) sham-operated (S; n = 8), 2) ovariectomized plus placebo (OVX; n = 8), 3) ovariectomized plus estradiol (OVX+E; n = 8), and 4) ovariectomized plus progesterone (OVX+P; n = 8). All ovariectomized groups were pair-fed to the S group. Body composition was measured using total body electrical conductivity. The relative increase in fat-free mass was greater (P < 0.01) in the OVX group (31 +/- 2%) than in the S (17 +/- 2%), OVX+E (18 +/- 2%), and OVX+P (22 +/- 2%) groups. The fractional synthetic rates of gastrocnemius muscle protein paralleled changes in fat-free mass: OVX had a higher (P < 0.05) synthesis rate (21 +/- 3%/day) than S (12 +/- 2%/day), OVX+E (11 +/- 2%/day), and OVX+P (8 +/- 1%/day) groups. Body fat increased in the S group (31 +/- 7%; P < 0.01), whereas the OVX groups lost fat (OVX: -10 +/- 7%; OVX+E: -15 +/- 7%; OVX+P: -13 +/- 7%). No differences in lipoprotein lipase were found. Our results suggest that estradiol and progesterone may regulate the growth of fat and fat-free tissues in female rats. Moreover, ovarian hormones may influence skeletal muscle growth through their effects on skeletal muscle protein synthesis.
Authors:
M J Toth; E T Poehlman; D E Matthews; A Tchernof; M J MacCoss
Related Documents :
12851515 - Isoflavone-rich soy protein prevents loss of hip lean mass but does not prevent the shi...
17495195 - Changes in body composition with weight loss: obese subjects randomized to surgical and...
476035 - A new method for measuring the body density of obese adults.
10409575 - Fat mass deposition during pregnancy using a four-component model.
10638295 - Effects of dietary substitution with raw and heat-treated cowpea (vigna unguiculata) on...
16414445 - Fermentable carbohydrate supplementation alters nitrogen excretion in chronic renal fai...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  280     ISSN:  0193-1849     ISO Abbreviation:  Am. J. Physiol. Endocrinol. Metab.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-03-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  E496-501     Citation Subset:  IM    
Affiliation:
Division of Clinical Pharmacology and Metabolic Research, Department of Medicine, University of Vermont, Burlington, VT 05405, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / drug effects,  enzymology*
Animals
Body Composition / drug effects*
Estradiol / pharmacology*
Female
Kinetics
Lipoprotein Lipase / metabolism*
Muscle Proteins / biosynthesis*
Muscle, Skeletal / drug effects,  metabolism
Ovariectomy
Progesterone / pharmacology*
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
AG-13978/AG/NIA NIH HHS; AM-02125/AM/NIADDK NIH HHS; RR-00109/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Muscle Proteins; 50-28-2/Estradiol; 57-83-0/Progesterone; EC 3.1.1.34/Lipoprotein Lipase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Generation of growth hormone pulsatility in women: evidence against somatostatin withdrawal as pulse...
Next Document:  [5-3H]glucose overestimates glycolytic flux in isolated working rat heart: role of the pentose phosp...