Document Detail

Effects of estradiol, phytoestrogens, and Ginkgo biloba extracts against 1-methyl-4-phenyl-pyridine-induced oxidative stress.
MedLine Citation:
PMID:  12777708     Owner:  NLM     Status:  MEDLINE    
Oxidative stress has been recently considered as a mediator of nerve cell death in several neurodegenerative diseases. We studied the effect of the parkinsonism-inducing toxine 1-methyl-4-phenyl-pyridine (MPP+) on several parameters of cell distress using native and neuronal PC12 cells. Then, since estrogens have been reported to prevent neuronal degeneration caused by oxidative damage, we investigated the ability of 17beta- estradiol (E2); two Ginkgo biloba extracts, EGb 761 and Cp 202; as well as two flavonoids, quercetin and kaempferol, to rescue PC12 cells submitted to MPP+- induced oxidative stress. Our results consistently show that both Ginkgo biloba extracts could prevent cell death in native and neuronal PC12 cells, while in neuronal PC12 cells also quercetin and E2 could reverse MPP+ neurotoxic effet. Western blot analysis demonstrated that MPP+ injuries might modulate dopamine transporter (DAT) protein expression but not estrogen receptor beta (ERbeta) protein expression. EGb 761 and Cp 202 also modulate DAT and ERbeta protein expression in neuronal cells. From these studies, we outline the importance of testing estrogen-like plant-derived molecules as potent antioxidants and examine their effect on protein expression.
Benoit Gagn?; Sylvie G?linas; Genevi?ve Bureau; Bruno Lagac?; Charles Ramassamy; Keith Chiasson; Barbara Valastro; Maria-Grazia Martinoli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrine     Volume:  21     ISSN:  1355-008X     ISO Abbreviation:  Endocrine     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-06-02     Completed Date:  2004-01-20     Revised Date:  2010-06-24    
Medline Journal Info:
Nlm Unique ID:  9434444     Medline TA:  Endocrine     Country:  United States    
Other Details:
Languages:  eng     Pagination:  89-95     Citation Subset:  IM    
Department of Biochemistry, Research Group in Neuroscience, Universit? du Qu?bec ? Trois-Rivi?res, Trois-Rivi?res, Canada.
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MeSH Terms
Blotting, Western
Cell Survival / drug effects
Dopamine Plasma Membrane Transport Proteins
Electrophoresis, Polyacrylamide Gel
Estradiol / pharmacology*
Estrogen Receptor beta
Ginkgo biloba*
Isoflavones / pharmacology*
Membrane Glycoproteins*
Membrane Transport Proteins / metabolism
Nerve Tissue Proteins*
Oxidative Stress / drug effects*,  physiology
PC12 Cells
Plant Extracts / pharmacology
Plant Preparations / pharmacology*
Pyridinium Compounds / pharmacology*
Quercetin / pharmacology
Receptors, Estrogen / biosynthesis,  drug effects,  genetics
Transcription, Genetic / drug effects
Reg. No./Substance:
0/Dopamine Plasma Membrane Transport Proteins; 0/Estrogen Receptor beta; 0/Isoflavones; 0/Membrane Glycoproteins; 0/Membrane Transport Proteins; 0/Nerve Tissue Proteins; 0/Phytoestrogens; 0/Plant Extracts; 0/Plant Preparations; 0/Pyridinium Compounds; 0/Receptors, Estrogen; 0/Slc6a3 protein, rat; 117-39-5/Quercetin; 42850-10-2/1-(4-methoxyphenyl)pyridinium; 50-28-2/Estradiol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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