Document Detail

Effects of enzymatic hydrolysis on lentil allergenicity.
MedLine Citation:
PMID:  20306474     Owner:  NLM     Status:  MEDLINE    
Enzymatic hydrolysis and further processing are commonly used to produce hypoallergenic dietary products derived from different protein sources, such as cow's milk. Lentils and chickpeas seem to be an important cause of IgE-mediated hypersensitivity in the Mediterranean area and India. Some studies have investigated the effects of enzymatic treatments on the in vitro immunological reactivity of members of the Leguminosae family, such as soybean, chickpea, lentil, and lupine. Nevertheless, there are only a few studies carried out to evaluate the effect on IgE reactivity of these food-hydrolysis products with sera from patients with well-documented allergy to these foods. In this study, lentil protein extract was hydrolyzed by sequential action of an endoprotease (Alcalase) and an exoprotease (Flavourzyme). Immunoreactivity to raw and hydrolyzed lentil extract was evaluated by means of IgE immunoblotting and ELISA using sera from five patients with clinical allergy to lentil. The results indicated that sequential hydrolysis of lentil results in an important proteolytic destruction of IgE-binding epitopes shown by in vitro experiments. However, some allergenic proteins were still detected by sera from four out of five patients in the last step of sequential hydrolyzation.
Beatriz Cabanillas; Mercedes M Pedrosa; Julia Rodríguez; Angela González; Mercedes Muzquiz; Carmen Cuadrado; Jesús F Crespo; Carmen Burbano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular nutrition & food research     Volume:  54     ISSN:  1613-4133     ISO Abbreviation:  Mol Nutr Food Res     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-08     Completed Date:  2011-01-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101231818     Medline TA:  Mol Nutr Food Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1266-72     Citation Subset:  IM    
Servicio de Alergia, Hospital Universitario 12 de Octubre, Madrid, Spain.
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MeSH Terms
Allergens / immunology*,  metabolism*
Endopeptidases / metabolism
Enzyme-Linked Immunosorbent Assay
Food Handling / methods
Food Hypersensitivity / blood,  immunology*
Immunoglobulin E / immunology,  metabolism
Lens Plant / immunology*,  metabolism
Plant Extracts / immunology,  metabolism
Plant Proteins / immunology*,  metabolism*
Protein Hydrolysates / immunology,  metabolism
Seeds / immunology*,  metabolism
Subtilisins / metabolism
Time Factors
Reg. No./Substance:
0/Allergens; 0/Plant Extracts; 0/Plant Proteins; 0/Protein Hydrolysates; 37341-29-0/Immunoglobulin E; EC 3.4.-/Endopeptidases; EC 3.4.-/flavourzyme; EC 3.4.21.-/Subtilisins

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