Document Detail

Effects of enteric bacterial and cyanobacterial lipopolysaccharides, and of microcystin-LR, on glutathione S-transferase activities in zebra fish (Danio rerio).
MedLine Citation:
PMID:  12200087     Owner:  NLM     Status:  MEDLINE    
Cyanobacteria (blue-green algae) can produce a variety of toxins including hepatotoxins e.g. microcystins, and endotoxins such as lipopolysaccharides (LPS). The combined effects of such toxins on fish are little known. This study examines the activities of microsomal (m) and soluble (s) glutathione S-transferases (GST) from embryos of the zebra fish, Danio rerio at the prim six embryo stage, which had been exposed since fertilisation to LPS from different sources. A further aim was to see how activity was affected by co-exposure to LPS and microcystin-LR (MC-LR). LPS were obtained from Salmonella typhimurium, Escherichia coli, a laboratory culture of Microcystis CYA 43 and natural cyanobacterial blooms of Microcystis and Gloeotrichia. Following in vivo exposure of embryos to each of the LPS preparations, mGST activity was significantly reduced (from 0.50 to between 0.06 and 0.32 nanokatals per milligram (nkat mg(-1)) protein). sGST activity in vivo was significantly reduced (from 1.05 to between 0.19 and 0.22 nkat mg(-1) protein) after exposure of embryos to each of the cyanobacterial LPS preparations, but not in response to S. typhimurium or E. coli LPS. Activities of both m- and sGSTs were reduced after co-exposure to MC-LR and cyanobacterial LPS, but only mGST activity was reduced in the S. typhimurium and E. coli LPS-treated embryos. In vitro preparations of GST from adult and prim six embryo D. rerio showed no significant changes in enzyme activity in response to the LPS preparations with the exception of Gloeotrichia bloom LPS, where mGST was reduced in adult and embryo preparations. The present study represents the first investigations into the effects of cyanobacterial LPS on the phase-II microcystin detoxication mechanism. LPS preparations, whether from axenic cyanobacteria or cyanobacterial blooms, are potentially capable of significantly reducing activity of both the s- and mGSTs, so reducing the capacity of D. rerio to detoxicate microcystins. The results presented here have wide ranging implications for both animal and human health.
J H Best; S Pflugmacher; C Wiegand; F B Eddy; J S Metcalf; G A Codd
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Aquatic toxicology (Amsterdam, Netherlands)     Volume:  60     ISSN:  0166-445X     ISO Abbreviation:  Aquat. Toxicol.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-08-29     Completed Date:  2002-10-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8500246     Medline TA:  Aquat Toxicol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  223-31     Citation Subset:  IM    
Division of Applied and Environmental Biology, School of Life Sciences, University of Dundee, DD1 4HN, Dundee, UK.
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MeSH Terms
Bacterial Toxins / pharmacokinetics,  toxicity
Cyanobacteria / metabolism
Enzyme Inhibitors / metabolism,  toxicity
Glutathione Transferase / metabolism*
Lipopolysaccharides / metabolism,  toxicity*
Metabolic Detoxication, Drug
Peptides, Cyclic / pharmacokinetics,  toxicity*
Zebrafish / metabolism*
Reg. No./Substance:
0/Bacterial Toxins; 0/Enzyme Inhibitors; 0/Lipopolysaccharides; 0/Microcystins; 0/Peptides, Cyclic; 101043-37-2/cyanoginosin LR; EC Transferase

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