Document Detail


Effects of enteric bacterial and cyanobacterial lipopolysaccharides, and of microcystin-LR, on glutathione S-transferase activities in zebra fish (Danio rerio).
MedLine Citation:
PMID:  12200087     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cyanobacteria (blue-green algae) can produce a variety of toxins including hepatotoxins e.g. microcystins, and endotoxins such as lipopolysaccharides (LPS). The combined effects of such toxins on fish are little known. This study examines the activities of microsomal (m) and soluble (s) glutathione S-transferases (GST) from embryos of the zebra fish, Danio rerio at the prim six embryo stage, which had been exposed since fertilisation to LPS from different sources. A further aim was to see how activity was affected by co-exposure to LPS and microcystin-LR (MC-LR). LPS were obtained from Salmonella typhimurium, Escherichia coli, a laboratory culture of Microcystis CYA 43 and natural cyanobacterial blooms of Microcystis and Gloeotrichia. Following in vivo exposure of embryos to each of the LPS preparations, mGST activity was significantly reduced (from 0.50 to between 0.06 and 0.32 nanokatals per milligram (nkat mg(-1)) protein). sGST activity in vivo was significantly reduced (from 1.05 to between 0.19 and 0.22 nkat mg(-1) protein) after exposure of embryos to each of the cyanobacterial LPS preparations, but not in response to S. typhimurium or E. coli LPS. Activities of both m- and sGSTs were reduced after co-exposure to MC-LR and cyanobacterial LPS, but only mGST activity was reduced in the S. typhimurium and E. coli LPS-treated embryos. In vitro preparations of GST from adult and prim six embryo D. rerio showed no significant changes in enzyme activity in response to the LPS preparations with the exception of Gloeotrichia bloom LPS, where mGST was reduced in adult and embryo preparations. The present study represents the first investigations into the effects of cyanobacterial LPS on the phase-II microcystin detoxication mechanism. LPS preparations, whether from axenic cyanobacteria or cyanobacterial blooms, are potentially capable of significantly reducing activity of both the s- and mGSTs, so reducing the capacity of D. rerio to detoxicate microcystins. The results presented here have wide ranging implications for both animal and human health.
Authors:
J H Best; S Pflugmacher; C Wiegand; F B Eddy; J S Metcalf; G A Codd
Related Documents :
25247417 - Identification of aminoglycoside and β-lactam resistance genes from within an infant g...
6497217 - Experimental subdural retrobulbar injection of anesthetic.
17095007 - Influence of nutrient deficiency caused by host developmental arrest on the growth and ...
15070957 - Inhibin b and anti-müllerian hormone, but not testosterone levels, are normal in infan...
913727 - The impact of breast feeding patterns on the biometric analysis of infant mortality.
17979027 - Activated protein c: controversy and hope in the treatment of sepsis.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Aquatic toxicology (Amsterdam, Netherlands)     Volume:  60     ISSN:  0166-445X     ISO Abbreviation:  Aquat. Toxicol.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-08-29     Completed Date:  2002-10-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8500246     Medline TA:  Aquat Toxicol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  223-31     Citation Subset:  IM    
Affiliation:
Division of Applied and Environmental Biology, School of Life Sciences, University of Dundee, DD1 4HN, Dundee, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Toxins / pharmacokinetics,  toxicity
Cyanobacteria / metabolism
Enzyme Inhibitors / metabolism,  toxicity
Female
Glutathione Transferase / metabolism*
Lipopolysaccharides / metabolism,  toxicity*
Male
Metabolic Detoxication, Drug
Microcystins
Peptides, Cyclic / pharmacokinetics,  toxicity*
Zebrafish / metabolism*
Chemical
Reg. No./Substance:
0/Bacterial Toxins; 0/Enzyme Inhibitors; 0/Lipopolysaccharides; 0/Microcystins; 0/Peptides, Cyclic; 101043-37-2/cyanoginosin LR; EC 2.5.1.18/Glutathione Transferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effects of long-term nonylphenol exposure on gonadal development and biomarkers of estrogenicity in ...
Next Document:  Seasonal variation of plasmatic and hepatic vitellogenin and EROD activity in carp, Cyprinus carpio,...