| Effects of endogenous insulin-like growth factor binding protein-3 on cell cycle regulation in breast cancer cells. | |
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MedLine Citation:
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PMID: 19675966 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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High tissue insulin-like growth factor binding protein-3 (IGFBP-3) expression in breast cancers is associated in some studies with rapid growth and poor outcome. This study examined the effects of endogenous IGFBP-3 in Hs578T breast cancer cells, which are IGF-unresponsive and grow aggressively despite relatively high IGFBP-3 expression. IGFBP-3 downregulation using siRNA was associated with increases in DNA synthesis, the percentage of cells in S phase and viable cell numbers, accompanied by increases in cyclins A and D1, a decrease in p27 expression, and increased phosphorylation of retinoblastoma (Rb) on Ser795. Downregulation of IGFBP-3 inhibited extracellular signal-regulated kinase (ERK) activation and cell migration in a monolayer wound healing assay. These results indicate that endogenous IGFBP-3 is anti-proliferative and pro-migratory in Hs578T cells and that these effects are IGF-independent. Poor outcome in breast tumours expressing high levels of IGFBP-3 may be due to the effects of IGFBP-3 on cell migration rather than cell growth. |
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Authors:
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Michelle K O'Han; Robert C Baxter; Lynette J Schedlich |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2009-8-12 |
Journal Detail:
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Title: Growth factors (Chur, Switzerland) Volume: - ISSN: 1029-2292 ISO Abbreviation: Growth Factors Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-8-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9000468 Medline TA: Growth Factors Country: - |
Other Details:
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Languages: ENG Pagination: 1 Citation Subset: - |
Affiliation:
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Kolling Institute of Medical Research, Royal North Shore Hospital, University of Sydney, Sydney, NSW, Australia. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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