Document Detail


Effects of employing a ¹⁰B-carrier and manipulating intratumour hypoxia on local tumour response and lung metastatic potential in boron neutron capture therapy.
MedLine Citation:
PMID:  22391496     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: To evaluate the effects of employing a (10)B-carrier and manipulating intratumour hypoxia on local tumour response and lung metastatic potential in boron neutron capture therapy (BNCT) by measuring the response of intratumour quiescent (Q) cells.
METHODS: B16-BL6 melanoma tumour-bearing C57BL/6 mice were continuously given 5-bromo-2'-deoxyuridine (BrdU) to label all proliferating (P) cells. The tumours received reactor thermal neutron beam irradiation following the administration of a (10)B-carrier [L-para-boronophenylalanine-(10)B (BPA) or sodium mercaptoundecahydrododecaborate-(10)B (BSH)] in combination with an acute hypoxia-releasing agent (nicotinamide) or mild temperature hyperthermia (MTH). Immediately after the irradiation, cells from some tumours were isolated and incubated with a cytokinesis blocker. The responses of the Q and total (P+Q) cell populations were assessed based on the frequency of micronuclei using immunofluorescence staining for BrdU. In other tumour-bearing mice, macroscopic lung metastases were enumerated 17 days after irradiation.
RESULTS: BPA-BNCT increased the sensitivity of the total tumour cell population more than BSH-BNCT. However, the sensitivity of Q cells treated with BPA was lower than that of BSH-treated Q cells. With or without a (10)B-carrier, MTH enhanced the sensitivity of the Q cell population. Without irradiation, nicotinamide treatment decreased the number of lung metastases. With irradiation, BPA-BNCT, especially in combination with nicotinamide treatment, showed the potential to reduce the number of metastases more than BSH-BNCT.
CONCLUSION: BSH-BNCT in combination with MTH improves local tumour control, while BPA-BNCT in combination with nicotinamide may reduce the number of lung metastases.
Authors:
S Masunaga; Y Sakurai; H Tanaka; M Suzuki; Y Liu; N Kondo; A Maruhashi; Y Kinashi; K Ono
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The British journal of radiology     Volume:  85     ISSN:  1748-880X     ISO Abbreviation:  Br J Radiol     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-06     Completed Date:  2012-04-23     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  0373125     Medline TA:  Br J Radiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  249-58     Citation Subset:  AIM; IM    
Affiliation:
Particle Radiation Oncology Research Centre, Research Reactor Institute, Kyoto University, Osaka, Japan. smasuna@rri.kyoto-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents / pharmacology*
Borohydrides / pharmacology*
Boron Neutron Capture Therapy / methods*
Bromodeoxyuridine / diagnostic use
Cell Hypoxia / drug effects
Female
Hyperthermia, Induced / methods*
Lung Neoplasms / prevention & control,  secondary
Melanoma, Experimental / pathology,  radiotherapy*
Mice
Mice, Inbred C57BL
Neoplasm Transplantation
Niacinamide / pharmacology
Radiation-Sensitizing Agents / pharmacology
Skin Neoplasms / radiotherapy*
Sulfhydryl Compounds / pharmacology*
Vitamin B Complex / pharmacology
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Borohydrides; 0/Radiation-Sensitizing Agents; 0/Sulfhydryl Compounds; 12001-76-2/Vitamin B Complex; 12294-22-3/mercaptoundecahydrododecaborate; 59-14-3/Bromodeoxyuridine; 98-92-0/Niacinamide
Comments/Corrections

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