Document Detail

Effects of electroacupuncture with different frequencies on spinal ionotropic glutamate receptor expression in complete Freund's adjuvant-injected rat.
MedLine Citation:
PMID:  15866287     Owner:  NLM     Status:  MEDLINE    
We investigated expressional changes of spinal glutamate receptors by electroacupuncture (EA) in an inflammatory animal model. Inflammation was induced by an intraplantar injection of complete Freund's adjuvant (CFA) into the hindpaw of male Sprague-Dawley rats. Bilateral EA stimulation at 2, 15 and 120 Hz was applied at those acupoints corresponding to Zusanli and Sanyinjiao in man using needles with 3-day intervals for 30 days. Paw edema and mechanical thresholds were measured by a water displacement plethysmometer and Analgesy-Meter, respectively. Edema and mechanical sensitivity of the hindpaw induced by CFA-injection were strongly inhibited by EA stimulation. At 30 days after CFA-injection, effects of EA on ionotropic glutamate receptor (NR-1, NR-2A, GluR-1 and GluR-2/3) expression in association with c-fos and calcitonin gene-related peptide (CGRP) expression were observed in the dorsal horn of the spinal cord using immunohistochemistry. The number of c-fos-like immunostained cells was decreased significantly in the superficial laminae of the dorsal horn by 2Hz EA, but CGRP expression also showed a marked decrease in the same region using the other types of EA stimulation. N-methyl-D-aspartate receptor (NR-1 and NR-2A) expression was attenuated in all regions of the dorsal horn by all types of EA. Of the alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor (GluR-1 and -2/3), only GluR-1 expression was prevented by EA treatment in the superficial laminae and the neck of the dorsal horn. It is concluded that EA treatment can attenuate inflammatory edema and mechanical thresholds in CFA-injected rats through modulating expression of ionotropic glutamate receptors, and especially N-methyl-D-aspartate receptors, in the dorsal horn of the spinal cord.
Byung-Tae Choi; Jin Kang; Un-Bock Jo
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta histochemica     Volume:  107     ISSN:  0065-1281     ISO Abbreviation:  Acta Histochem.     Publication Date:  2005  
Date Detail:
Created Date:  2005-05-03     Completed Date:  2005-09-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370320     Medline TA:  Acta Histochem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  67-76     Citation Subset:  IM    
Department of Anatomy, College of Oriental Medicine, Dong-Eui University, Busan 614-052, Republic of Korea.
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MeSH Terms
Calcitonin Gene-Related Peptide / biosynthesis
Cell Count
Edema / chemically induced,  pathology,  therapy*
Electroacupuncture* / methods
Freund's Adjuvant / administration & dosage,  adverse effects*
Inflammation / chemically induced,  therapy*
Injections, Spinal
Pain Measurement
Pain Threshold
Proto-Oncogene Proteins c-fos / biosynthesis
Rats, Sprague-Dawley
Receptors, Glutamate / analysis,  biosynthesis*,  ultrastructure
Spinal Cord / chemistry,  metabolism*,  ultrastructure
Reg. No./Substance:
0/Proto-Oncogene Proteins c-fos; 0/Receptors, Glutamate; 83652-28-2/Calcitonin Gene-Related Peptide; 9007-81-2/Freund's Adjuvant

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