Document Detail

Effects of eccentric exercise-induced muscle injury on blood levels of platelet activating factor (PAF) and other inflammatory markers.
MedLine Citation:
PMID:  16151835     Owner:  NLM     Status:  MEDLINE    
It has been reported that exercise with eccentric contractions can induce damage and inflammation in human muscle tissue, the severity of which depends on the duration and the intensity of exercise. Platelet activating factor (PAF) is a potent inflammatory mediator implicated in a series of pathophysiological conditions. We sought to investigate the relationship between PAF and eccentric exercise induced muscle damage. Thirteen healthy, recreationally active male subjects (27.5+/-3.78 year) performed 36 maximum voluntary eccentric contractions on a motorized muscle dynamometer using the elbow flexor muscles of the non-dominant arm. Venous blood samples were collected immediately before and after exercise at 2, 24, 48, 72 and 96 h. PAF was isolated, purified and determined by a platelet aggregation assay. Serum levels of creatine kinase (CK), lactate dehydrogenase (LDH), C-reactive protein (CRP) and complement C3 (C3), plasma level of fibrinogen and whole blood level of leukocytes (and their subsets) were determined. Established indicators of muscle damage as maximum isometric torque (MIT), range of motion (ROM), relaxed arm angle (RANG), flexed arm angle (FANG), arm circumference (CIRC) and muscle soreness were also measured at the same time points. PAF, leukocytes, CK and LDH were elevated after exercise, while other biochemical parameters such as CRP, C3 and fibrinogen were unchanged. We also observed an inverse association between PAF and MIT and ROM, as well as a positive association with other markers of muscle injury, i.e. CK, LDH, FANG and CIRC (all P's<0.05). Our findings may imply a role for PAF in the mechanism of eccentric exercise induced muscle damage.
George A Milias; Tzortzis Nomikos; Elizabeth Fragopoulou; Spyridon Athanasopoulos; Smaragdi Antonopoulou
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Publication Detail:
Type:  Journal Article     Date:  2005-09-06
Journal Detail:
Title:  European journal of applied physiology     Volume:  95     ISSN:  1439-6319     ISO Abbreviation:  Eur. J. Appl. Physiol.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-05     Completed Date:  2006-03-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100954790     Medline TA:  Eur J Appl Physiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  504-13     Citation Subset:  IM    
Department of Science of Nutrition - Dietetics, Harokopio University of Athens, 70 El. Venizelou str., 176 71, Athens, Greece.
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MeSH Terms
C-Reactive Protein / metabolism
Complement C3 / metabolism
Creatine Kinase / blood
Elbow Joint / physiology
Exercise / physiology*
Fibrinogen / metabolism
Inflammation / metabolism
L-Lactate Dehydrogenase / blood
Leukocyte Count
Muscle Contraction / physiology*
Muscle, Skeletal / injuries*,  physiology
Platelet Activating Factor / metabolism*
Reg. No./Substance:
0/Complement C3; 0/Platelet Activating Factor; 9001-32-5/Fibrinogen; 9007-41-4/C-Reactive Protein; EC Dehydrogenase; EC Kinase

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