| Effects of dopamine and L-DOPA on survival of PC12 cells. | |
| | |
MedLine Citation:
|
PMID: 11002293 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The effects of dopamine and L-DOPA on survival were examined in differentiated PC12 cells. Addition of dopamine to the culture medium at 3-30 microM prevented cell death induced by depletion of serum and nerve growth factor (NGF). At 100 microM, dopamine induced cell death. The cell-protective effect of dopamine was not affected by nomifensine, an inhibitor of dopamine uptake, or pargyline, an inhibitor of monoamine oxidase, suggesting that dopamine is working outside the cell. The cell-protective effect of dopamine was blunted by SCH-23390, a D(1) antagonist, but not sulpiride, a D(2) antagonist, indicating that the cell protective effect of dopamine is mediated by D(1) receptors in PC12 cells. L-DOPA also protected PC12 cells from cell death induced by depletion of serum and NGF at low concentrations and showed toxicity at high concentration. The effect of L-DOPA was unchanged after inhibition of conversion of L-DOPA to dopamine by m-hydroxybenzylhydrazine (NSD-1015), an inhibitor of DOPA decarboxylase, suggesting that L-DOPA itself is working for cell protection. Intracellular Ca(2+) concentration and mitogen-activated protein (MAP) kinase activity were increased by both dopamine and L-DOPA. The effects of dopamine and L-DOPA on cell survival were blunted by nicardipine, a Ca(2+) channel blocker, and PD-98059, an inhibitor of MAP kinase kinase (MEK). These results taken together raised the possibility that dopamine and L-DOPA protect PC12 cells from cell death at low concentrations by activating MAP kinase activity via elevation of intracellular Ca(2+) concentration. |
| | |
Authors:
|
K Koshimura; J Tanaka; Y Murakami; Y Kato |
Related Documents
:
|
14758223 - Human embryonic kidney cells (hek-293 cells): characterization and dose-response relati... 15686493 - Identification of autotaxin as a neurite retraction-inducing factor of pc12 cells in ce... 187973 - An in vitro study of the oncogenic effects of two variants of avian sarcoma virus b77 o... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of neuroscience research Volume: 62 ISSN: 0360-4012 ISO Abbreviation: J. Neurosci. Res. Publication Date: 2000 Oct |
Date Detail:
|
Created Date: 2000-10-23 Completed Date: 2000-11-30 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 7600111 Medline TA: J Neurosci Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 112-9 Citation Subset: IM |
Copyright Information:
|
Copyright 2000 Wiley-Liss, Inc. |
Affiliation:
|
First Division, Department of Medicine, Shimane Medical University, Izumo, Japan. kunio@shimane-med.ac.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Calcium / metabolism Calcium Channel Blockers / pharmacology Cell Differentiation / drug effects Cell Survival / drug effects Culture Media, Serum-Free / pharmacology Dopa Decarboxylase / antagonists & inhibitors Dopamine / pharmacology* Dopamine Uptake Inhibitors / pharmacology Dose-Response Relationship, Drug Flavonoids / pharmacology Intracellular Fluid / metabolism Levodopa / pharmacology* Mitogen-Activated Protein Kinase Kinases / antagonists & inhibitors Mitogen-Activated Protein Kinases / metabolism Monoamine Oxidase Inhibitors / pharmacology Nerve Growth Factor / pharmacology Neuroprotective Agents / pharmacology PC12 Cells Rats Receptors, Dopamine D1 / antagonists & inhibitors |
| Chemical | |
Reg. No./Substance:
|
0/Calcium Channel Blockers; 0/Culture Media, Serum-Free; 0/Dopamine Uptake Inhibitors; 0/Flavonoids; 0/Levodopa; 0/Monoamine Oxidase Inhibitors; 0/Neuroprotective Agents; 0/PD 98059; 0/Receptors, Dopamine D1; 7440-70-2/Calcium; 9061-61-4/Nerve Growth Factor; EC 2.7.11.24/Mitogen-Activated Protein Kinases; EC 2.7.12.2/Mitogen-Activated Protein Kinase Kinases; EC 4.1.1.-/Dopa Decarboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: 7beta-hydroxysterol is cytotoxic to neonatal rat astrocytes in primary culture when cAMP levels are ...
Next Document: Prion peptide fragment PrP[106-126] forms distinct cation channel types.