Document Detail

Effects of docosahexaenoic and arachidonic acids on the synthesis and distribution of aminophospholipids during neuronal differentiation of PC12 cells.
MedLine Citation:
PMID:  10087166     Owner:  NLM     Status:  MEDLINE    
We have shown previously that docosahexaenoic acid (DHA) promotes and arachidonic acid (AA) suppresses neurite outgrowth of PC12 cells induced by nerve growth factor (NGF) and that incorporation of [3H]ethanolamine into phosphatidylethanolamine (PE) is suppressed in PC12 cells by AA while DHA has no effect. In the present study, the effects of these fatty acids on PE synthesis via decarboxylation of phosphatidylserine (PS), another pathway of PE synthesis, and distribution of aminophospholipids were examined. Incorporation of [3H]serine into PS and PE was elevated in the course of NGF-induced differentiation and was further stimulated significantly by DHA, but not by AA. [3H]Ethanolamine uptake by PC12 cells was significantly suppressed by AA but not by DHA while these fatty acids did not affect [3H]serine uptake, indicating that the suppression by AA of [3H]ethanolamine incorporation into phosphatidylethanolamine is attributable, at least in part, to a reduction in [3H]ethanolamine uptake. The distribution of PE in the outer leaflet of plasma membrane decreased during differentiation, which is known to be accompanied by an increase in the surface area of plasma membrane. Supplementation of PC12 cells with DHA or AA did not affect the distribution of aminophospholipids. Thus, DHA and AA affected aminophospholipid synthesis and neurite outgrowth differently, but not the transport and distribution of aminophospholipids, while the PE concentration in the outer leaflet of the plasma membrane decreased in association with morphological changes in PC12 cells induced by NGF.
A Ikemoto; T Kobayashi; K Emoto; M Umeda; S Watanabe; H Okuyama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  364     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-05-21     Completed Date:  1999-05-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  67-74     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Faculty of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabedori, Nagoya, Mizuhoku, 467-8603, Japan.
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MeSH Terms
Anti-Bacterial Agents / metabolism
Arachidonic Acid / pharmacology*
Cell Differentiation
Cell Membrane / metabolism
Cell Size / drug effects
Docosahexaenoic Acids / pharmacology*
Ethanolamine / metabolism
Flow Cytometry
Hemolysin Proteins / metabolism
Nerve Growth Factors / pharmacology
Neurons / drug effects,  metabolism*
PC12 Cells
Phosphatidylethanolamines / metabolism
Phosphatidylserines / metabolism
Phospholipids / metabolism*
Serine / metabolism
Trinitrobenzenesulfonic Acid / metabolism
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Hemolysin Proteins; 0/Nerve Growth Factors; 0/Peptides; 0/Phosphatidylethanolamines; 0/Phosphatidylserines; 0/Phospholipids; 10028-17-8/Tritium; 110655-58-8/lanthiopeptin; 141-43-5/Ethanolamine; 2508-19-2/Trinitrobenzenesulfonic Acid; 25167-62-8/Docosahexaenoic Acids; 506-32-1/Arachidonic Acid; 56-45-1/Serine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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