| Effects of docosahexaenoic acid on mouse brain synaptic plasma membrane proteome analyzed by mass spectrometry and (16)O/(18)O labeling. | |
| | |
MedLine Citation:
|
PMID: 22003853 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Docosahexenoic acid (DHA, 22:6n-3) plays an important role in development of proper brain function in mammals. We have previously reported that DHA promotes synaptogenesis and synaptic function in hippocampal neurons while DHA-depletion in the brain due to n-3 fatty acid deficiency produces opposite effects. To gain insight into underlying molecular mechanisms, we investigated whether the brain DHA status affects the synaptic plasma membrane (SPM) proteome by using nanoLC-ESI-MS/MS and (16)O/(18)O labeling. The DHA level in mouse brains was lowered by dietary depletion of n-3 fatty acids, and SPM was prepared by differential centrifugation followed by osmotic shock. SPM proteins from DHA-adequate and depleted brains were analyzed by nanoLC-ESI-MS/MS after SDS-PAGE, in-gel digestion, and differential O(18)/O(16) labeling. This strategy allowed comparative quantitation of more than 200 distinct membrane or membrane-associated proteins from DHA-adequate or depleted brains. We found that 18 pre- and postsynaptic proteins that are relevant to synaptic physiology were significantly down-regulated in DHA-depleted mouse brains. The protein network analysis suggests involvement of CREB and caspase-3 pathways in the DHA-dependent modulation of synaptic proteome. Reduction of specific synaptic proteins due to brain DHA-depletion may be an important mechanism for the suboptimal brain function associated with n-3 fatty acid deficiency. |
| | |
Authors:
|
Vishaldeep K Sidhu; Bill X Huang; Hee-Yong Kim |
Related Documents
:
|
21164573 - Characterization of pseudomonas aeruginosa fatty acid profiles in biofilms and batch pl... 21159423 - Insights into cadmium induced physiological and ultra-structural disorders in juncus ef... 7370283 - Clofibrate enhancement of mitochondrial carnitine transport system of rat liver and aug... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, N.I.H., Intramural Date: 2011-10-26 |
Journal Detail:
|
Title: Journal of proteome research Volume: 10 ISSN: 1535-3907 ISO Abbreviation: J. Proteome Res. Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2011-12-05 Completed Date: 2012-03-25 Revised Date: 2013-05-23 |
Medline Journal Info:
|
Nlm Unique ID: 101128775 Medline TA: J Proteome Res Country: United States |
Other Details:
|
Languages: eng Pagination: 5472-80 Citation Subset: IM |
Affiliation:
|
Laboratory of Molecular Signaling, Division of Intramural Clinical and Biological Reasearch, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, Maryland, United States. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blotting, Western Centrifugation / methods Cerebral Cortex / chemistry, drug effects* Cyclic AMP Response Element-Binding Protein / chemistry Docosahexaenoic Acids / pharmacology* Down-Regulation Electrophoresis, Polyacrylamide Gel Fatty Acids, Omega-3 / chemistry Female Isotope Labeling / methods* Mass Spectrometry / methods Membrane Proteins / analysis, chemistry Mice Mice, Inbred C57BL Osmotic Pressure Oxygen Isotopes / chemistry Pregnancy Proteome / analysis*, chemistry Synapses / chemistry Synaptic Membranes / chemistry, drug effects* |
| Grant Support | |
ID/Acronym/Agency:
|
ZIA AA000284-21/AA/NIAAA NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Creb1 protein, mouse; 0/Cyclic AMP Response Element-Binding Protein; 0/Fatty Acids, Omega-3; 0/Membrane Proteins; 0/Oxygen Isotopes; 0/Proteome; 25167-62-8/Docosahexaenoic Acids |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Heat to connect: Surface commensurability directs organometallic one-dimensional self-assembly.
Next Document: Sleepwalking through History: Medicine, Arts, and Courts of Law.