Document Detail


Effects of digoxin-specific antibody Fab fragment (Digibind) on blood pressure and renal water-sodium metabolism in 5/6 reduced renal mass hypertensive rats.
MedLine Citation:
PMID:  10371371     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The importance of increased endogenous digitalis-like factor (EDLF) in volume-expanded hypertension has been generally agreed. To further clarify the role of EDLF on the development of hypertension and renal water-sodium handling in 5/6 reduced renal mass hypertensive rats (RRM), we studied the effects of acute administration of digoxin-specific antibody Fab fragment (Digibind) in the early phase and the chronic phase of hypertension in RRM. RRM and sham-operated rats were given 1% saline for 1 or 4 weeks. RRM were injected Digibind (60 mg/kg) or vehicle (0.9% saline) intravenously in the first or fourth week under thiobutabarbital anesthesia. All sham-operated rats were administered Digibind under the same condition. Digibind altered neither blood pressure, heart rate, urine volume, nor urinary sodium excretion in sham-operated rats. However, Digibind produced a gradual but significant decline in mean arterial pressure to the level slightly above that in sham-operated rats from 153 +/- 5 to 131 +/- 5 mm Hg in the first week and from 181 +/- 6 to 129 +/- 4 mm Hg in the fourth week without any significant change in heart rate. The decrease in mean arterial pressure at 160 min after Digibind administration in the fourth week (-48 +/- 5 mm Hg) was greater than that in the first week (-22 +/- 4 mm Hg). No differences were observed in urine volume, urinary sodium excretion, or plasma norepinephrine concentration between Digibind and vehicle-treated RRM in either week. These data suggest that EDLF would contribute to both the early and chronic phase in the development of hypertension in RRM.
Authors:
J Kaide; N Ura; T Torii; M Nakagawa; T Takada; K Shimamoto
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of hypertension     Volume:  12     ISSN:  0895-7061     ISO Abbreviation:  Am. J. Hypertens.     Publication Date:  1999 Jun 
Date Detail:
Created Date:  1999-08-02     Completed Date:  1999-08-02     Revised Date:  2009-02-24    
Medline Journal Info:
Nlm Unique ID:  8803676     Medline TA:  Am J Hypertens     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  611-9     Citation Subset:  IM    
Affiliation:
Second Department of Internal Medicine, Sapporo Medical University, School of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Pressure / drug effects*
Body Water / metabolism
Cardenolides
Digoxin / immunology*
Heart Rate / drug effects
Hypertension, Renal / metabolism*
Immunoglobulin Fab Fragments / administration & dosage,  pharmacology*
Injections, Intravenous
Kidney / drug effects,  metabolism*,  physiology
Male
Norepinephrine / blood
Rats
Rats, Sprague-Dawley
Renal Circulation / drug effects
Saponins / metabolism*
Sodium / urine*
Chemical
Reg. No./Substance:
0/Cardenolides; 0/Immunoglobulin Fab Fragments; 0/Saponins; 0/digoxin antibodies Fab fragments; 0/digoxin-like factors; 20830-75-5/Digoxin; 51-41-2/Norepinephrine; 7440-23-5/Sodium
Comments/Corrections
Comment In:
Am J Hypertens. 2000 May;13(5 Pt 1):569-70   [PMID:  10826414 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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